Antibodies, Proteins, Kits and Reagents for Life Science

291 484 ₸ Product size

100 µl 291 484 ₸

Order now and get it on Thursday March 04, 2021

Anti-ERp72 antibody [EPR14824(B)] (ab190348)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR14824(B)] to ERp72
Suitable for: WB, IHC-P, Flow Cyt
Reacts with: Human

Product name

Anti-ERp72 antibody [EPR14824(B)]
See all ERp72 primary antibodies
Description

Rabbit monoclonal [EPR14824(B)] to ERp72
Host species

Rabbit

Tested Applications & Species

Application	Species
Flow Cyt	Human
IHC-P	Human
WB	Human

See all applications and species data

Immunogen

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
Positive control
- HeLa, JAR and MCF7 cell lysates; Human fetal liver lysate. Human lung carcinoma tissue. MCF7 cells.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR14824(B)
Isotype

IgG
Research areas

Western blot - Anti-ERp72 antibody [EPR14824(B)] (ab190348)

All lanes : Anti-ERp72 antibody [EPR14824(B)] (ab190348) at 1/10000 dilution

Lane 1 : HeLa cell lysate
Lane 2 : JAR cell lysate
Lane 3 : Human fetal liver lysate
Lane 4 : MCF7 cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : goat anti-rabbit IgG, (H+L), peroxidase conjugated at 1/1000 dilution

Developed using the ECL technique.

Predicted band size: 73 kDa
Observed band size: 73 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERp72 antibody [EPR14824(B)] (ab190348)

Immunohistochemical analysis of paraffin-embedded, Human lung carcinoma tissue labeling ERp72 with ab190348 at a 1/100 dilution. Counter stained with hematoxylin. Negative control also shown.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Flow Cytometry - Anti-ERp72 antibody [EPR14824(B)] (ab190348)

Flow cytometry analysis of 2% paraformaldehyde-fixed MCF7 cells labeling ERp72 with ab190348 at a 1/50 dilution (pink) or a rabbit IgG isotype control (green), followed by secondary Goat anti rabbit IgG (FITC) at 1/150 dilution.
Anti-ERp72 antibody [EPR14824(B)] (ab190348)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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