Astana Biomed Group, an authorized Abcam distributor in Central Asia

ErbB2 / HER2 Antibody Panel (EP1045Y, EPR19547-12, SP3, SP101, CAL27) (ab252204)

Overview

  • Product name

    ErbB2 / HER2 Antibody Panel (EP1045Y, EPR19547-12, SP3, SP101, CAL27)
    See all ErbB2 / HER2 kits

  • Species reactivity

    Reacts with: Human

  • Product overview

    ErbB2 / HER2 Antibody Panel ab252204 contains multiple trial-sized versions of anti-human antibody clones against ErbB2 / HER2, specifically selected for high performance in IHC. This panel contains 5 recombinant rabbit monoclonal antibodies against human ErbB2 / HER2. They are provided in trial sizes to allow you to easily evaluate which is the best ErbB2 / HER2 antibody for your IHC research assay needs.


    For guidelines on how to use each antibody within the panel, please consult the individual datasheet for each antibody.


    Panel contains:


    - Rabbit monoclonal [CAL27] to ErbB2 / HER2 (20 µL) ab237715


    - Rabbit monoclonal [EP1045Y] to ErbB2 / HER2 (20 µL) ab134182


    - Rabbit monoclonal [EPR19547-12] to ErbB2 / HER2 (20 µL) ab214275


    - Rabbit monoclonal [SP101] to ErbB2 / HER2 (20 µL) ab231438


    - Rabbit monoclonal [SP3] to ErbB2 / HER2 (20 µL) ab16662

  • Notes

    Explore our range of antibody sample panels designed to provide you with a variety of trial-size antibodies in a convenient and cost-effective format.

    Carrier-free formulations of our recombinant antibodies are available and ready to use for multiplex IHC analysis including Imaging Mass CytometryTM. Please refer to the ‘Associated products’ section below.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Storage instructions

    Store at -20°C. Please refer to protocols.
  • Components 1 kit
    ab237715 - Anti-ErbB 2 antibody [CAL27] 2 x 10µl
    ab134182 - Anti-ErbB 2 antibody [EP1045Y] 2 x 10µl
    ab214275 - Anti-ErbB 2 antibody [EPR19547-12] 2 x 10µl
    ab231438 - Anti-ErbB 2 antibody [SP101] 2 x 10µl
    ab16662 - Anti-ErbB 2 antibody [SP3] 2 x 10µl

  • Research areas

  • Function

    Protein tyrosine kinase that is part of several cell surface receptor complexes, but that apparently needs a coreceptor for ligand binding. Essential component of a neuregulin-receptor complex, although neuregulins do not interact with it alone. GP30 is a potential ligand for this receptor. Regulates outgrowth and stabilization of peripheral microtubules (MTs). Upon ERBB2 activation, the MEMO1-RHOA-DIAPH1 signaling pathway elicits the phosphorylation and thus the inhibition of GSK3B at cell membrane. This prevents the phosphorylation of APC and CLASP2, allowing its association with the cell membrane. In turn, membrane-bound APC allows the localization of MACF1 to the cell membrane, which is required for microtubule capture and stabilization.
    In the nucleus is involved in transcriptional regulation. Associates with the 5'-TCAAATTC-3' sequence in the PTGS2/COX-2 promoter and activates its transcription. Implicated in transcriptional activation of CDKN1A; the function involves STAT3 and SRC. Involved in the transcription of rRNA genes by RNA Pol I and enhances protein synthesis and cell growth.

  • Tissue specificity

    Expressed in a variety of tumor tissues including primary breast tumors and tumors from small bowel, esophagus, kidney and mouth.

  • Involvement in disease

    Hereditary diffuse gastric cancer
    Glioma
    Ovarian cancer
    Lung cancer
    Gastric cancer
    Chromosomal aberrations involving ERBB2 may be a cause gastric cancer. Deletions within 17q12 region producing fusion transcripts with CDK12, leading to CDK12-ERBB2 fusion leading to truncated CDK12 protein not in-frame with ERBB2.

  • Sequence similarities

    Belongs to the protein kinase superfamily. Tyr protein kinase family. EGF receptor subfamily.
    Contains 1 protein kinase domain.

  • Post-translational
    modifications

    Autophosphorylated. Autophosphorylation occurs in trans, i.e. one subunit of the dimeric receptor phosphorylates tyrosine residues on the other subunit (Probable). Ligand-binding increases phosphorylation on tyrosine residues (PubMed:27134172). Signaling via SEMA4C promotes phosphorylation at Tyr-1248 (PubMed:17554007). Dephosphorylated by PTPN12 (PubMed:27134172).

  • Cellular localization

    Cytoplasm. Nucleus and Cell membrane. Cytoplasm, perinuclear region. Nucleus. Translocation to the nucleus requires endocytosis, probably endosomal sorting and is mediated by importin beta-1/KPNB1.
  • Information by UniProt

  • Alternative names

    • Verb b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog
    • C erb B2/neu protein
    • CD340
    • CD340 antigen
    • Cerb B2/neu protein
    • CerbB2
    • Erb b2 receptor tyrosine kinase 2
    • ErbB-2 proto-oncogene
    • ERBB2
    • ERBB2_HUMAN
    • HER 2
    • HER 2/NEU
    • HER2
    • Herstatin
    • Human epidermal growth factor receptor 2
    • Metastatic lymph node gene 19 protein
    • MLN 19
    • MLN19
    • NEU
    • NEU proto oncogene
    • Neuro/glioblastoma derived oncogene homolog
    • Neuroblastoma/glioblastoma derived oncogene homolog
    • NGL
    • p185erbB2
    • Proto-oncogene c-ErbB-2
    • Proto-oncogene Neu
    • Receptor tyrosine-protein kinase erbB-2
    • TKR1
    • Tyrosine kinase type cell surface receptor HER2
    • Tyrosine kinase-type cell surface receptor HER2
    • V erb b2 avian erythroblastic leukemia viral oncogene homolog 2
    • V erb b2 avian erythroblastic leukemia viral oncogene homolog 2 (neuro/glioblastoma derived oncogene homolog)
    • V erb b2 avian erythroblastic leukemia viral oncoprotein 2
    • V erb b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog
    • V erb b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog (avian)
    • Verb b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog (avian)
    see all

  • Database links

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ErbB 2 [SP3]
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ErbB 2 [SP3]

      Formalin-fixed, paraffin-embedded human breast, invasive ductal carcinoma tissue stained for ErbB2 / HER2 using ab16662 at 1/200 dilution in immunohistochemical analysis, followed by Goat anti Rabbit IgG HRP.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ErbB 2 [EPR19547-12]
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ErbB 2 [EPR19547-12]

      Immunohistochemical analysis of paraffin-embedded Human breast cancer tissue labeling ErbB2 / HER2 with ab214275 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

      Membrane staining on Human breast cancer is observed [PMID: 18437174].

      Counter stained with Hematoxylin.

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab97051 at 1/500 dilution.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ErbB 2 [SP101]
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ErbB 2 [SP101]

      Formalin-fixed, paraffin-embedded human breast carcinoma tissue stained for ErbB2 / HER2 using ab231438 at 1/100 dilution in immunohistochemical analysis.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ErbB 2 [EP1045Y]
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ErbB 2 [EP1045Y]

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human breast carcinoma tissue sections labeling ErbB2 / HER2 with Purified ab134182 at 1:1600 dilution (0.68 µg/ml). Heat mediated antigen retrieval was performed using Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0). Tissue was counterstained with Hematoxylin. ImmunoHistoProbe one step HRP Polymer (ready to use) secondary antibody was used at 1:0 dilution. PBS instead of the primary antibody was used as the negative control.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ErbB 2 [CAL27]
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ErbB 2 [CAL27]

      Formalin-fixed, paraffin-embedded human breast carcinoma tissue stained for ErbB2 / HER2 using ab237715  at 0.3 μg/ml in immunohistochemical analysis.

    • ErbB2 / HER2 Antibody Panel (EP1045Y, EPR19547-12, SP3, SP101, CAL27) (ab252204)
      ErbB2 / HER2 Antibody Panel (EP1045Y, EPR19547-12, SP3, SP101, CAL27) (ab252204)

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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