Antibodies, Proteins, Kits and Reagents for Life Science

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CD8 alpha Antibody Panel (EP1150Y, SP16, SP239, CAL66, CAL67) (ab252196)

Product name

CD8 alpha Antibody Panel (EP1150Y, SP16, SP239, CAL66, CAL67)
See all CD8 alpha kits
Species reactivity

Reacts with: Human
Product overview

CD8 alpha Antibody Panel ab252196 contains multiple trial-sized versions of anti-human antibody clones against CD8 alpha, specifically selected for high performance in IHC. This panel contains 6 recombinant rabbit monoclonal antibodies against human CD8 alpha. They are provided in trial sizes to allow you to easily evaluate which is the best CD8 alpha antibody for your IHC research assay needs.

For guidelines on how to use each antibody within the panel, please consult the individual datasheet for each antibody.

Panel contains:

- Rabbit monoclonal [CAL66] to CD8 alpha (20 µL) ab237709

- Rabbit monoclonal [CAL67] to CD8 alpha (20 µL) ab237710

- Rabbit monoclonal [EP1150Y] to CD8 alpha (20 µL) ab93278

- Rabbit monoclonal [SP16] to CD8 alpha (20 µL) ab101500

- Rabbit monoclonal [SP239] to CD8 alpha (20 µL) ab178089
Notes

Explore our range of antibody sample panels designed to provide you with a variety of trial-size antibodies in a convenient and cost-effective format.

Carrier-free formulations of our recombinant antibodies are available and ready to use for multiplex IHC analysis including Imaging Mass Cytometry^TM. Please refer to the ‘Associated products’ section below.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Storage instructions

Store at -20°C. Please refer to protocols.

Components	1 kit
ab237709 - Anti-CD8 alpha antibody [CAL66]	2 x 10µl
ab237710 - Anti-CD8 alpha antibody [CAL67]	2 x 10µl
ab93278 - Anti-CD8 alpha antibody [EP1150Y]	2 x 10µl
ab101500 - Anti-CD8 alpha antibody [SP16]	2 x 10µl
ab178089 - Anti-CD8 alpha antibody [SP239]	2 x 10µl

Research areas
Function

Identifies cytotoxic/suppressor T-cells that interact with MHC class I bearing targets. CD8 is thought to play a role in the process of T-cell mediated killing. CD8 alpha chains binds to class I MHC molecules alpha-3 domains.
Involvement in disease

Defects in CD8A are a cause of familial CD8 deficiency (CD8 deficiency) [MIM:608957]. Familial CD8 deficiency is a novel autosomal recessive immunologic defect characterized by absence of CD8+ cells, leading to recurrent bacterial infections.
Sequence similarities

Contains 1 Ig-like V-type (immunoglobulin-like) domain.
Post-translational
modifications

All of the five most carboxyl-terminal cysteines form inter-chain disulfide bonds in dimers and higher multimers, while the four N-terminal cysteines do not.
Cellular localization

Secreted and Cell membrane.
Target information above from: UniProt accession P01732 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
Alternative names
- alpha polypeptide (p32)
- CD_antigen=CD8a
- CD8
- CD8 antigen alpha polypeptide
- CD8 antigen alpha polypeptide (p32)
- CD8 antigen, alpha polypeptide (p32)
- CD8a
- CD8A antigen
- CD8A molecule
- CD8A_HUMAN
- Leu2
- Leu2 T lymphocyte antigen
- Ly 2
- Ly 35
- Ly B
- Ly2
- Ly3
- Ly35
- LyB
- Lyt 2.1 lymphocyte differentiation antigen (AA at 100)
- LYT3
- MAL
- OKT8 T cell antigen
- OTTHUMP00000160760
- OTTHUMP00000160764
- OTTHUMP00000203528
- OTTHUMP00000203721
- p32
- T cell antigen Leu2
- T cell co receptor
- T lymphocyte differentiation antigen T8/Leu 2
- T-cell surface glycoprotein CD8 alpha chain
- T-cell surface glycoprotein Lyt 2
- T-lymphocyte differentiation antigen T8/Leu-2
- T8 T cell antigen
- T8/Leu-2 T-lymphocyte differentiation antigen
see all
Database links
- Entrez Gene: 925 Human
- Omim: 186910 Human
- SwissProt: P01732 Human
- Unigene: 85258 Human

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD8 alpha antibody [SP239]

Immunohistochemical analysis of formalin-fixed, paraffin-embedded human tonsil tissue labeling CD8 alpha with ab178089 at 1/100 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD8 alpha antibody [SP16]

Immunohistochemical staining of paraffin embedded Human tonsil tissue labelling CD8 alpha [SP16] with ab101500 at 1/100.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD8 alpha antibody [EP1150Y]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human spleen tissue labelling CD8 alpha with purified ab93278 at 1/500. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD8 alpha antibody [CAL67]

Formalin-fixed, paraffin-embedded human tonsil tissue stained for CD8 alpha using ab237710 at 0.5 μg/ml in immunohistochemical analysis.

Incubate with primary antibody for 75 minutes at room temperature.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD8 alpha antibody [CAL66]

Formalin-fixed, paraffin-embedded human tonsil tissue stained for CD8 alpha using ab237709 at 0.25 µg/ml in immunohistochemical analysis.
CD8 alpha Antibody Panel (EP1150Y, SP16, SP239, CAL66, CAL67) (ab252196)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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