CD8 alpha Antibody Panel (EPR21769, EPR20305, EP1150, EPR22483-288, EPR10640(2)) (ab252197)
Overview
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Product name
CD8 alpha Antibody Panel (EPR21769, EPR20305, EP1150, EPR22483-288, EPR10640(2))
See all CD8 alpha kits -
Species reactivity
Reacts with: Human -
Product overview
CD8 alpha Antibody Panel ab252197 contains multiple trial-sized versions of anti-human antibody clones against CD8 alpha, specifically selected for high performance in IHC. This panel contains 5 recombinant rabbit monoclonal antibodies against human CD8 alpha. They are provided in trial sizes to allow you to easily evaluate which is the best CD8 alpha antibody for your IHC research assay needs.
For guidelines on how to use each antibody within the panel, please consult the individual datasheet for each antibody.
Panel contains:
- Rabbit monoclonal [EP1150] to CD8 alpha (20 µL) ab108343
- Rabbit monoclonal [EPR20305] to CD8 alpha (20 µL) ab209775
- Rabbit monoclonal [EPR21769] to CD8 alpha (20 µL) ab217344
- Rabbit monoclonal [EPR22483-288] to CD8 alpha (20 µL) ab245118
- Rabbit monoclonal [EPR10640(2)] to CD8 alpha - N-terminal (20 µL) ab189926
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Notes
Explore our range of antibody sample panels designed to provide you with a variety of trial-size antibodies in a convenient and cost-effective format.
Carrier-free formulations of our recombinant antibodies are available and ready to use for multiplex IHC analysis including Imaging Mass CytometryTM. Please refer to the ‘Associated products’ section below.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 kit ab108343 - Anti-CD8 alpha antibody [EP1150] 2 x 10µl ab189926 - Anti-CD8 alpha antibody [EPR10640(2)] - N-terminal 2 x 10µl ab209775 - Anti-CD8 alpha antibody [EPR20305] 2 x 10µl ab217344 - Anti-CD8 alpha antibody [EPR21769] 2 x 10µl ab245118 - Anti-CD8 alpha antibody [EPR22483-288] 2 x 10µl -
Research areas
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Function
Identifies cytotoxic/suppressor T-cells that interact with MHC class I bearing targets. CD8 is thought to play a role in the process of T-cell mediated killing. CD8 alpha chains binds to class I MHC molecules alpha-3 domains. -
Involvement in disease
Defects in CD8A are a cause of familial CD8 deficiency (CD8 deficiency) [MIM:608957]. Familial CD8 deficiency is a novel autosomal recessive immunologic defect characterized by absence of CD8+ cells, leading to recurrent bacterial infections. -
Sequence similarities
Contains 1 Ig-like V-type (immunoglobulin-like) domain. -
Post-translational
modificationsAll of the five most carboxyl-terminal cysteines form inter-chain disulfide bonds in dimers and higher multimers, while the four N-terminal cysteines do not. -
Cellular localization
Secreted and Cell membrane. - Information by UniProt
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Alternative names
- alpha polypeptide (p32)
- CD_antigen=CD8a
- CD8
see all -
Database links
- Entrez Gene: 925 Human
- Omim: 186910 Human
- SwissProt: P01732 Human
- Unigene: 85258 Human
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD8 alpha antibody [EPR10640(2)]
Immunohistochemical analysis of paraffin-embedded, Human tonsil tissue tissue labeling CD8 alpha with ab189926 at a 1/500 dilution. Counter stained with hematoxylin. Negative control also shown.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD8 alpha antibody [EPR22483-288]
Immunohistochemical analysis of paraffin-embedded human tonsil tissue labeling CD8 alpha with ab245118 at 1/1000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Positive staining on the T cell zone of human tonsil (PMID: 16504163) is observed. Counterstained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).
Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0). -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD8 alpha antibody [EPR21769]
Immunohistochemical analysis of paraffin-embedded mouse spleen tissue labeling CD8 alpha with ab217344 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Positive staining on the white pulp of mouse spleen (PMID: 23482450; PMID: 25826597). Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD8 alpha antibody [EPR20305]
Immunohistochemical analysis of paraffin-embedded mouse spleen tissue labeling CD8 alpha with ab209775 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Membranous staining on T cells around peripheral artery lymphatic sheath of mouse spleen (PMID: 23482450). Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD8 alpha antibody [EP1150]
ab108343 at 1/250 dilution staining CD8 alpha in Human tonsil tissue by Immunohistochemistry Formalin-fixed, Paraffin-embedded tissue.
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