c-Met Antibody Panel (EP1454Y, EPR19067, SP44, CAL12) (ab252205)
Overview
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Product name
c-Met Antibody Panel (EP1454Y, EPR19067, SP44, CAL12)
See all Met (c-Met) kits -
Species reactivity
Reacts with: Human -
Product overview
c-met Antibody Panel ab252205 contains multiple trial-sized versions of anti-human antibody clones against c-Met, specifically selected for high performance in IHC. This panel contains 4 recombinant rabbit monoclonal antibodies against human c-Met. They are provided in trial sizes to allow you to easily evaluate which is the best c-Met antibody for your IHC research assay needs.
For guidelines on how to use each antibody within the panel, please consult the individual datasheet for each antibody.
Panel contains:
- Rabbit monoclonal [CAL12] to Met (c-Met) (20 µL) ab237711
- Rabbit monoclonal [EP1454Y] to Met (c-Met) - N-terminal (20 µL) ab51067
- Rabbit monoclonal [EPR19067] to Met (c-Met) (20 µL) ab216574
- Rabbit monoclonal [SP44] to Met (c-Met) - C-terminal (20 µL) ab227637
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Notes
Explore our range of antibody sample panels designed to provide you with a variety of trial-size antibodies in a convenient and cost-effective format.
Carrier-free formulations of our recombinant antibodies are available and ready to use for multiplex IHC analysis including Imaging Mass CytometryTM. Please refer to the ‘Associated products’ section below.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 kit ab237711 - Anti-Met (c-Met) antibody [CAL12] 2 x 10µl ab51067 - Anti-Met (c-Met) antibody [EP1454Y] - N-terminal 2 x 10µl ab216574 - Anti-Met (c-Met) antibody [EPR19067] 2 x 10µl ab227637 - Anti-Met (c-Met) antibody [SP44] - C-terminal 2 x 10µl -
Research areas
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Function
Receptor for hepatocyte growth factor and scatter factor. Has a tyrosine-protein kinase activity. Functions in cell proliferation, scattering, morphogenesis and survival. -
Involvement in disease
Note=Activation of MET after rearrangement with the TPR gene produces an oncogenic protein.
Note=Defects in MET may be associated with gastric cancer.
Defects in MET are a cause of hepatocellular carcinoma (HCC) [MIM:114550].
Defects in MET are a cause of renal cell carcinoma papillary (RCCP) [MIM:605074]. It is a subtype of renal cell carcinoma tending to show a tubulo-papillary architecture formed by numerous, irregular, finger-like projections of connective tissue. Renal cell carcinoma is a heterogeneous group of sporadic or hereditary carcinoma derived from cells of the proximal renal tubular epithelium. It is subclassified into common renal cell carcinoma (clear cell, non-papillary carcinoma), papillary renal cell carcinoma, chromophobe renal cell carcinoma, collecting duct carcinoma with medullary carcinoma of the kidney, and unclassified renal cell carcinoma.
Note=A common allele in the promoter region of the MET shows genetic association with susceptibility to autism in some families. Functional assays indicate a decrease in MET promoter activity and altered binding of specific transcription factor complexes.
Note=MET activating mutations may be involved in the development of a highly malignant, metastatic syndrome known as cancer of unknown primary origin (CUP) or primary occult malignancy. Systemic neoplastic spread is generally a late event in cancer progression. However, in some instances, distant dissemination arises at a very early stage, so that metastases reach clinical relevance before primary lesions. Sometimes, the primary lesions cannot be identified in spite of the progresses in the diagnosis of malignancies. -
Sequence similarities
Belongs to the protein kinase superfamily. Tyr protein kinase family.
Contains 3 IPT/TIG domains.
Contains 1 protein kinase domain.
Contains 1 Sema domain. -
Domain
The kinase domain is involved in SPSB1 binding. -
Post-translational
modificationsDephosphorylated by PTPRJ at Tyr-1349 and Tyr-1365. -
Cellular localization
Membrane. - Information by UniProt
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Alternative names
- AUTS9
- c met
- D249
see all -
Database links
- Entrez Gene: 4233 Human
- Omim: 164860 Human
- SwissProt: P08581 Human
- Unigene: 132966 Human
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-c-Met [EP1454Y]
ab51067 staining Met (c-Met) in human breast tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections).
Tissue was fixed with formaldehyde, permeabilized with 0.05% Tween-20 and blocked for 30 minutes at 22°C; antigen retrieval was by heat mediation in antigen retrieval buffer (100X citrate buffer pH 6.0) (ab94674). Samples were incubated with the primary antibody (1/100) for 14 hours at 4°C. An Alexa Fluor® 488-conjugated goat anti-rabbit IgG polyclonal (1/300) was used as the secondary antibody.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-c-Met [CAL12]](https://www.abcam.com/ps/products/252/ab252205/Images/ab252205-342973-cmetantibodypanel-cal12-Immunohistochemistryparaffin-lungcarcinoma-human.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-c-Met [CAL12]Formalin-fixed, paraffin-embedded human non-small cell lung carcinoma tissue stained for Met (c-Met) using ab237711 at 1 μg/ml in immunohistochemical analysis.
Incubate with primary antibody for 75 minutes at room temperature.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-c-Met [SP44]](https://www.abcam.com/ps/products/252/ab252205/Images/ab252205-342976-cmetantibodypanel-sp44-Immunohistochemistryparaffin-colonadenocarcinoma-human.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-c-Met [SP44]Formalin-fixed, paraffin-embedded human colon adenocarcinoma tissue stained for Met (c-Met) using ab227637 at 1/50 dilution in immunohistochemical analysis.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-c-Met [EPR19067]](https://www.abcam.com/ps/products/252/ab252205/Images/ab252205-342975-cmetantibodypanel-epr19067-Immunohistochemistryparaffin-breasttissue-human.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-c-Met [EPR19067]Immunohistochemical analysis of paraffin-embedded human breast tissue labeling Met (c-Met) with ab216574 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use.
Membranous staining on human breast is observed.
Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.
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c-Met Antibody Panel (EP1454Y, EPR19067, SP44, CAL12) (ab252205)
