T Cell Exhaustion Marker (PD1, CTLA4, TIM3, LAG3, TIGIT) Antibody Panel - Human, IHC (ab254018)
Overview
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Product name
T Cell Exhaustion Marker (PD1, CTLA4, TIM3, LAG3, TIGIT) Antibody Panel - Human, IHC -
Species reactivity
Reacts with: Human -
Product overview
T Cell Exhaustion Marker (PD1, CTLA4, TIM3, LAG3, TIGIT) Antibody Panel - Human, IHC ab254018 contains multiple trial-sized versions of anti-human antibody clones against PD1, CTLA4, TIM3, LAG3, TIGIT, specifically selected for high performance in IHC. This panel contains 4 recombinant rabbit monoclonal antibodies against human CTLA4, TIM3, LAG3, TIGIT and 1 mouse monoclonal antibody against human PD1. They are provided as a sampler panel to allow you to easily evaluate each in your required applications.
For guidelines on how to use each antibody within the panel, please consult the individual datasheet for each antibody.
Panel contains:
- Mouse monoclonal [NAT105] to PD1 (20 µg) ab52587
- Rabbit monoclonal [CAL49] to CTLA4 (20 µL) ab237712
- Rabbit monoclonal [EPR22241] to TIM 3 (20 µL) ab241332
- Rabbit monoclonal [EPR20261] to LAG-3 (20 µL) ab209236
- Rabbit monoclonal [BLR047F] to TIGIT (20 µL) ab243903
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Notes
Explore our range of antibody sample panels designed to provide you with a variety of trial-size antibodies in a convenient and cost-effective format.
Carrier-free formulations of our recombinant antibodies are available and ready to use for multiplex IHC analysis including Imaging Mass CytometryTM. Please refer to the ‘Associated products’ section below.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Storage instructions
Please refer to protocols. -
Components 1 kit ab237712 - Anti-CTLA4 antibody [CAL49] 2 x 10µl ab209236 - Anti-Lymphocyte Activation Gene 3 antibody [EPR20261] 2 x 10µl ab52587 - Anti-PD1 antibody [NAT105] (ab52587) 1 x 20µg ab243903 - Anti-TIGIT antibody [BLR047F] 2 x 10µl ab241332 - Anti-TIM 3 antibody [EPR22241] 2 x 10µl -
Research areas
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Cellular localization
CTLA4: Cell membrane. Exists primarily an intracellular antigen whose surface expression is tightly regulated by restricted trafficking to the cell surface and rapid internalisation and. TIM 3: Membrane. Cell junction. Localizes to the immunological synapse between CD8+ T-cells and target cells. PD1: Membrane. Lymphocyte Activation Gene 3: Membrane. TIGIT: Cell membrane. -
Database links
- Entrez Gene: 1493 Human
- Entrez Gene: 201633 Human
- Entrez Gene: 3902 Human
- Entrez Gene: 5133 Human
- Entrez Gene: 84868 Human
- Omim: 123890 Human
- Omim: 153337 Human
- Omim: 600244 Human
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PD1 antibody [NAT105]
Immunohistochemical analysis of paraffin-embedded human tonsil tissue labeling PD1 with ab52587 at 1/50 dilution, followed by Rabbit Anti-Mouse IgG + Anti-Rabbit IgG H&L (HRP) Ready to use. Positive staining on T cells of human tonsil germinal center is observed. Performed on a Leica Biosystems BOND instrument. Counter stained with hematoxylin.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CTLA4 antibody [CAL49]](https://www.abcam.com/ps/products/254/ab254018/Images/ab254018-344475-T-cell-exhaustion-marker-antibody-panel-CAL49-immunohistochemistry-breast-carcinoma-human.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CTLA4 antibody [CAL49]Formalin-fixed, paraffin-embedded human breast carcinoma tissue stained for CTLA4 using ab237712 at 0.25 μg/ml in immunohistochemical analysis.
Incubate with primary antibody for 75 minutes at room temperature.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TIM 3 antibody [EPR22241]](https://www.abcam.com/ps/products/254/ab254018/Images/ab254018-344476-T-cell-exhaustion-marker-antibody-panel-EPR22241-immunohistochemistry-lung-cancer-human.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TIM 3 antibody [EPR22241]Immunohistochemical analysis of paraffin-embedded human lung cancer tissue labeling TIM 3 with ab241332 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Cytoplasmic and membranous staining on both infiltrated immunocytes and tumor cells of human lung cancer (PMID: 22586058) is observed. Counter stained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.
Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TIM 3 antibody [EPR22241]](https://www.abcam.com/ps/products/254/ab254018/Images/ab254018-344477-T-cell-exhaustion-marker-antibody-panel-EPR22241-immunohistochemistry-liver-human.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TIM 3 antibody [EPR22241]Immunohistochemical analysis of paraffin-embedded human liver tissue labeling TIM 3 with ab241332 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Cytoplasmic and membranous staining on Kupffer cells of human liver (PMID: 27192565) is observed. Counter stained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.
Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lymphocyte Activation Gene 3 antibody [EPR20261]](https://www.abcam.com/ps/products/254/ab254018/Images/ab254018-344478-T-cell-exhaustion-marker-antibody-panel-EPR20261-immunohistochemistry-tonsil-hodgkins-lymphoma-human.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lymphocyte Activation Gene 3 antibody [EPR20261]Immunohistochemical analysis of paraffin-embedded human tonsil Hodgkin’s lymphoma labeling Lymphocyte Activation Gene 3 with ab209236 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Cytoplasmic staining on immunocytes of the human Hodgkin’s lymphoma [PMID: 11527700; PMID: 16757686].
Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lymphocyte Activation Gene 3 antibody [EPR20261]](https://www.abcam.com/ps/products/254/ab254018/Images/ab254018-344479-T-cell-exhaustion-marker-antibody-panel-EPR20261-immunohistochemistry-tonsil-human.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lymphocyte Activation Gene 3 antibody [EPR20261]Immunohistochemical analysis of paraffin-embedded human tonsil tissue labeling Lymphocyte Activation Gene 3 with ab209236 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Sporadic cytoplasmic staining on immunocytes of human tonsil [PMID: 11527700; PMID: 16757686].
Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TIGIT antibody [BLR047F]](https://www.abcam.com/ps/products/254/ab254018/Images/ab254018-344480-T-cell-exhaustion-marker-antibody-panel-BLR047F-immunohistochemistry-tonsil-human.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TIGIT antibody [BLR047F]Formalin-fixed, paraffin-embedded human tonsil tissue stained for TIGIT using ab243903 at 1/200 dilution in immunohistochemical analysis. A HRP-conjugated goat anti-rabbit antibody was used as the secondary. DAB staining.
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T Cell Exhaustion Marker (PD1, CTLA4, TIM3, LAG3, TIGIT) Antibody Panel - Human, IHC (ab254018)
