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Immunology Adaptive Immunity T Cells CD

LAG-3 Antibody Panel (EPR20261, EPR4392(2), SP346, CAL77, CAL25, CAL26) (ab252193)

Price and availability

348 441 ₸

Availability

Order now and get it on Tuesday March 09, 2021

LAG-3 Antibody Panel (EPR20261, EPR4392(2), SP346, CAL77, CAL25, CAL26) (ab252193)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

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Overview

  • Product name

    LAG-3 Antibody Panel (EPR20261, EPR4392(2), SP346, CAL77, CAL25, CAL26)
    See all LAG-3 kits
  • Species reactivity

    Reacts with: Human
  • Product overview

    Lymphocyte Activation Gene 3 Antibody Panel ab252193 contains multiple trial-sized versions of anti-human antibody clones against Lymphocyte Activation Gene 3, specifically selected for high performance in IHC. This panel contains 6 recombinant rabbit monoclonal antibodies against human Lymphocyte Activation Gene 3. They are provided in trial sizes to allow you to easily evaluate which is the best Lymphocyte Activation Gene 3 antibody for your IHC research assay needs.


    For guidelines on how to use each antibody within the panel, please consult the individual datasheet for each antibody.


    Panel contains:


     - Rabbit monoclonal [CAL25] to Lymphocyte Activation Gene 3 (20 µL) ab237718


    - Rabbit monoclonal [CAL26] to Lymphocyte Activation Gene 3 (20 µL) ab237719


    - Rabbit monoclonal [CAL77] to Lymphocyte Activation Gene 3 (20 µL) ab237720


    - Rabbit monoclonal [EPR20261] to Lymphocyte Activation Gene 3 (20 µL) ab209236


    - Rabbit monoclonal [EPR4392(2)] to Lymphocyte Activation Gene 3 - C-terminal (20 µL) ab180187


    - Rabbit monoclonal [SP346] to Lymphocyte Activation Gene 3 - C-terminal (20 µL) ab227706

  • Notes

    Explore our range of antibody sample panels designed to provide you with a variety of trial-size antibodies in a convenient and cost-effective format.

    Carrier-free formulations of our recombinant antibodies are available and ready to use for multiplex IHC analysis including Imaging Mass CytometryTM. Please refer to the ‘Associated products’ section below.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Storage instructions

    Store at -20°C. Please refer to protocols.
  • Components 1 kit
    ab237718 - Anti-Lymphocyte Activation Gene 3 antibody [CAL25] 2 x 10µl
    ab237719 - Anti-Lymphocyte Activation Gene 3 antibody [CAL26] 2 x 10µl
    ab237720 - Anti-Lymphocyte Activation Gene 3 antibody [CAL77] 2 x 10µl
    ab209236 - Anti-Lymphocyte Activation Gene 3 antibody [EPR20261] 2 x 10µl
    ab180187 - Anti-Lymphocyte Activation Gene 3 antibody [EPR4392(2)] - C-terminal 2 x 10µl
    ab227706 - Anti-Lymphocyte Activation Gene 3 antibody [SP346] - C-terminal 2 x 10µl
  • Research areas

    • Immunology
    • Adaptive Immunity
    • T Cells
    • CD
    • Immunology
    • Cell Type Markers
    • CD
    • NK Cells
    • Immunology
    • Secreted Molecules
    • Other secreted molecules
    • Immunology
    • Adaptive Immunity
    • Regulatory T Cells
  • Function

    Involved in lymphocyte activation. Binds to HLA class-II antigens.
  • Tissue specificity

    On cell surface of activated NK and T-lymphocytes.
  • Sequence similarities

    Contains 3 Ig-like C2-type (immunoglobulin-like) domains.
    Contains 1 Ig-like V-type (immunoglobulin-like) domain.
  • Cellular localization

    Membrane.
  • Target information above from: UniProt accession P18627 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Alternative names

    • CD223
    • CD223 antigen
    • FDC protein
    • LAG-3
    • Lag3
    • LAG3_HUMAN
    • Lymphocyte activating 3
    • Lymphocyte activation gene 3
    • Lymphocyte activation gene 3 protein
    • Protein FDC
    see all
  • Database links

    • Entrez Gene: 3902 Human
    • Omim: 153337 Human
    • SwissProt: P18627 Human
    • Unigene: 409523 Human

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAG-3 antibody [SP346]
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAG-3 antibody [SP346]

      Formalin-fixed, paraffin-embedded human tonsil tissue stained for Lymphocyte Activation Gene 3 using ab227706 at 1/100 dilution in immunohistochemical analysis.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAG-3 antibody [EPR4392(2)]
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAG-3 antibody [EPR4392(2)]

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human brain tissue sections labeling Lymphocyte Activation Gene 3 with ab180187 at 1/8000 dilution (0.13 µg/mL). Tissue was pre-treated with Tris EDTA buffer, pH 9. Detection was carried out using ab209101, Rabbit specific IHC polymer detection kit HRP/DAB. Micrographs were captured using the Leica AperioAT2 using the 20x objective.

      Lymphocyte Activation Gene 3 staining in gray matter. Neuron cell bodies are strongly labeled and moderate staining in neuropil.

       

       

       

       

       

       

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAG-3 antibody [EPR20261]
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAG-3 antibody [EPR20261]

      Immunohistochemical analysis of paraffin-embedded human tonsil Hodgkin’s lymphoma labeling Lymphocyte Activation Gene 3 with ab209236 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Cytoplasmic staining on immunocytes of the human Hodgkin’s lymphoma [PMID: 11527700; PMID: 16757686].

      Counter stained with Hematoxylin.

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAG-3 antibody [CAL77]
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAG-3 antibody [CAL77]

      Lymphocyte Activation Gene 3 transfected HEK-293 (Human epithelial cell line from embryonic kidney) cells stained for Lymphocyte Activation Gene 3 with ab237720 at 0.25 µg/ml in immunocytochemical analysis.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAG-3 antibody [CAL26]
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAG-3 antibody [CAL26]

      Formalin-fixed, paraffin-embedded human tonsil tissue stained for Lymphocyte Activation Gene 3 using ab237719 at 0.3 μg/ml in immunohistochemical analysis.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAG-3 antibody [CAL25]
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAG-3 antibody [CAL25]

      Formalin-fixed, paraffin-embedded human tonsil tissue stained for Lymphocyte Activation Gene 3 using ab237718 at 0.3 μg/ml in immunohistochemical analysis.

    • LAG-3 Antibody Panel (EPR20261, EPR4392(2), SP346, CAL77, CAL25, CAL26) (ab252193)
      LAG-3 Antibody Panel (EPR20261, EPR4392(2), SP346, CAL77, CAL25, CAL26) (ab252193)

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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