LAG-3 Antibody Panel (EPR20261, EPR4392(2), SP346, CAL77, CAL25, CAL26) (ab252193)
Overview
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Product name
LAG-3 Antibody Panel (EPR20261, EPR4392(2), SP346, CAL77, CAL25, CAL26)
See all LAG-3 kits -
Species reactivity
Reacts with: Human -
Product overview
Lymphocyte Activation Gene 3 Antibody Panel ab252193 contains multiple trial-sized versions of anti-human antibody clones against Lymphocyte Activation Gene 3, specifically selected for high performance in IHC. This panel contains 6 recombinant rabbit monoclonal antibodies against human Lymphocyte Activation Gene 3. They are provided in trial sizes to allow you to easily evaluate which is the best Lymphocyte Activation Gene 3 antibody for your IHC research assay needs.
For guidelines on how to use each antibody within the panel, please consult the individual datasheet for each antibody.
Panel contains:
- Rabbit monoclonal [CAL25] to Lymphocyte Activation Gene 3 (20 µL) ab237718
- Rabbit monoclonal [CAL26] to Lymphocyte Activation Gene 3 (20 µL) ab237719
- Rabbit monoclonal [CAL77] to Lymphocyte Activation Gene 3 (20 µL) ab237720
- Rabbit monoclonal [EPR20261] to Lymphocyte Activation Gene 3 (20 µL) ab209236
- Rabbit monoclonal [EPR4392(2)] to Lymphocyte Activation Gene 3 - C-terminal (20 µL) ab180187
- Rabbit monoclonal [SP346] to Lymphocyte Activation Gene 3 - C-terminal (20 µL) ab227706
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Notes
Explore our range of antibody sample panels designed to provide you with a variety of trial-size antibodies in a convenient and cost-effective format.
Carrier-free formulations of our recombinant antibodies are available and ready to use for multiplex IHC analysis including Imaging Mass CytometryTM. Please refer to the ‘Associated products’ section below.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Research areas
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Function
Involved in lymphocyte activation. Binds to HLA class-II antigens. -
Tissue specificity
On cell surface of activated NK and T-lymphocytes. -
Sequence similarities
Contains 3 Ig-like C2-type (immunoglobulin-like) domains.
Contains 1 Ig-like V-type (immunoglobulin-like) domain. -
Cellular localization
Membrane. - Information by UniProt
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Alternative names
- CD223
- CD223 antigen
- FDC protein
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Database links
- Entrez Gene: 3902 Human
- Omim: 153337 Human
- SwissProt: P18627 Human
- Unigene: 409523 Human
Images
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Formalin-fixed, paraffin-embedded human tonsil tissue stained for Lymphocyte Activation Gene 3 using ab227706 at 1/100 dilution in immunohistochemical analysis.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAG-3 antibody [EPR4392(2)]
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human brain tissue sections labeling Lymphocyte Activation Gene 3 with ab180187 at 1/8000 dilution (0.13 µg/mL). Tissue was pre-treated with Tris EDTA buffer, pH 9. Detection was carried out using ab209101, Rabbit specific IHC polymer detection kit HRP/DAB. Micrographs were captured using the Leica AperioAT2 using the 20x objective.
Lymphocyte Activation Gene 3 staining in gray matter. Neuron cell bodies are strongly labeled and moderate staining in neuropil.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAG-3 antibody [EPR20261]
Immunohistochemical analysis of paraffin-embedded human tonsil Hodgkin’s lymphoma labeling Lymphocyte Activation Gene 3 with ab209236 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Cytoplasmic staining on immunocytes of the human Hodgkin’s lymphoma [PMID: 11527700; PMID: 16757686].
Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.
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Lymphocyte Activation Gene 3 transfected HEK-293 (Human epithelial cell line from embryonic kidney) cells stained for Lymphocyte Activation Gene 3 with ab237720 at 0.25 µg/ml in immunocytochemical analysis.
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Formalin-fixed, paraffin-embedded human tonsil tissue stained for Lymphocyte Activation Gene 3 using ab237719 at 0.3 μg/ml in immunohistochemical analysis.
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Formalin-fixed, paraffin-embedded human tonsil tissue stained for Lymphocyte Activation Gene 3 using ab237718 at 0.3 μg/ml in immunohistochemical analysis.
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