Anti-CD1c antibody [OTI2F4] (ab156708)
![Anti-CD1c antibody [OTI2F4] (ab156708)](https://www.abcam.com/ps/products/156/ab156708/Images/ab156708-163813-anti-cd1c-antibody-oti2f4-immunohistochemistry.jpg "Anti-CD1c antibody [OTI2F4] (ab156708)")
Key features and details
- Mouse monoclonal [OTI2F4] to CD1c
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG1
Overview
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Product name
Anti-CD1c antibody [OTI2F4]
See all CD1c primary antibodies -
Description
Mouse monoclonal [OTI2F4] to CD1c -
Host species
Mouse -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P HumanWB Human
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Immunogen
Full length human recombinant protein of human CD1C(NP_001756) produced in HEK293T cell.
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General notes
The clone number has been updated from 2F4 to OTI2F4, both clone numbers name the same clone.
This product was changed from ascites to tissue culture supernatant on 29th May 2018. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: PBS, 1% BSA, 50% Glycerol -
Concentration information loading... -
Purity
Protein G purified -
Purification notes
Purified from TCS -
Clonality
Monoclonal -
Clone number
OTI2F4 -
Isotype
IgG1 -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD1c antibody [OTI2F4] (ab156708)Immunohistochemical staining of paraffin-embedded Human lymph node tissue using anti-CD1C mouse monoclonal antibody(ab156708)
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![Western blot - Anti-CD1c antibody [OTI2F4] (ab156708)](https://www.abcam.com/ps/products/156/ab156708/Images/ab156708-163812-anti-cd1c-antibody-oti2f4-western-blot.jpg)
Western blot - Anti-CD1c antibody [OTI2F4] (ab156708)All lanes : Anti-CD1c antibody [OTI2F4] (ab156708)
Lane 1 : HEK293T cells were transfected with the pCMV6-ENTRY
Lane 2 : HEK293T cells were transfected with pCMV6-ENTRY CD1C
Lysates/proteins at 5 µg per lane.
Predicted band size: 38 kDa
HEK293T cell lysates were generated from transient transfection of the cDNA clone (RC211378) -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD1c antibody [OTI2F4] (ab156708)](https://www.abcam.com/ps/products/156/ab156708/Images/ab156708-277445-anti-cd1c-antibody-oti2f4-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD1c antibody [OTI2F4] (ab156708) This image is courtesy of an Abreview submitted by Jing Maab156708 staining MAFA in Cynomolgus monkey lymph node sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 10% donkey serum for 20 minutes at romm temperature; antigen retrieval was by heat mediation. Samples were incubated with primary antibody (1/50) for 30 minutes at room temperature. A Biotin-conjugated Donkey anti-mouse IgG polyclonal (1/2000) was used as the secondary antibody.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD1c antibody [OTI2F4] (ab156708)](https://www.abcam.com/ps/products/156/ab156708/Images/ab156708-163811-anti-cd1c-antibody-oti2f4-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD1c antibody [OTI2F4] (ab156708)Immunohistochemical staining of paraffin-embedded Human tonsil using anti-CD1C mouse monoclonal antibody. (ab156708)
