Anti-CD8 beta antibody [EPR22331-54] - BSA and Azide free (ab254197)
![Anti-CD8 beta antibody [EPR22331-54] - BSA and Azide free (ab254197)](https://www.abcam.com/ps/products/254/ab254197/Images/ab254197-4-228965IP1.jpg "Anti-CD8 beta antibody [EPR22331-54] - BSA and Azide free (ab254197)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR22331-54] to CD8 beta - BSA and Azide free
- Suitable for: WB, IHC-P, IHC-Fr, IP
- Reacts with: Mouse
Overview
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Product name
Anti-CD8 beta antibody [EPR22331-54] - BSA and Azide free
See all CD8 beta primary antibodies -
Description
Rabbit monoclonal [EPR22331-54] to CD8 beta - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, IHC-Fr, IPmore details
Unsuitable for: Flow Cyt -
Species reactivity
Reacts with: Mouse -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- IP: Mouse thymus lysate. IHC-P: Mouse spleen and thymus tissue. IHC-Fr: Mouse thymus tissue.
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General notes
Ab254197 is the carrier-free version of ab228965. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab254197 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR22331-54 -
Isotype
IgG -
Research areas
Images
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Immunoprecipitation - Anti-CD8 beta antibody [EPR22331-54] - BSA and Azide free (ab254197)
CD8 beta was immunoprecipitated from 0.35 mg of mouse thymus lysate with ab228965 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab228965 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used as secondary antibody at 1/5000 dilution.
Lane 1: Mouse thymus lysate 10 μg (Input).
Lane 2: ab228965 IP in mouse thymus lysate.
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab22896 in mouse thymus lysate.Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 3 seconds.This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab228965).
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![Immunohistochemistry (Frozen sections) - Anti-CD8 beta antibody [EPR22331-54] - BSA and Azide free (ab254197)](https://www.abcam.com/ps/products/254/ab254197/Images/ab254197-3-228965IHCFr1.jpg)
Immunohistochemistry (Frozen sections) - Anti-CD8 beta antibody [EPR22331-54] - BSA and Azide free (ab254197)Frozen section of 4% PFA-fixed, 0.2% Triton X-100 permeabilized mouse thymus tissue stained for CD8 beta (green) using ab228965 at 1/100 dilution in immunihistochemical analysis. The secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) at 1/1000 dilution. Specific membrane staining on T cells in the cortex of mouse thymus (PMID 12702501) is observed. The nuclear conter stain is DAPI (blue).
Secondary antibody only control: Used PBS instead of primary antibody, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) at 1/1000 dilution.
Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab228965).
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD8 beta antibody [EPR22331-54] - BSA and Azide free (ab254197)](https://www.abcam.com/ps/products/254/ab254197/Images/ab254197-2-228965IHC2.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD8 beta antibody [EPR22331-54] - BSA and Azide free (ab254197)Immunohistochemical analysis of paraffin-embedded mouse thymus tissue labeling CD8 beta with ab228965 at 1/500 dilution, followed by Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on T cells in the cortex of mouse thymus (PMID: 12702501) is observed. Counter stained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 minutes.
The section was incubated with ab228965 for 30 mins at RT. The immunostaining was performed on a Leica Biosystems BOND® RX instrument.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab228965).
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD8 beta antibody [EPR22331-54] - BSA and Azide free (ab254197)](https://www.abcam.com/ps/products/254/ab254197/Images/ab254197-1-228965IHC1.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD8 beta antibody [EPR22331-54] - BSA and Azide free (ab254197)Immunohistochemical analysis of paraffin-embedded mouse spleen tissue labeling CD8 beta with ab228965 at 1/500 dilution, followed by Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on T cells in periarterial lymphatic sheath of mouse spleen (PMID: 18776164) is observed. Counter stained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 minutes.
The section was incubated with ab228965 for 30 mins at RT. The immunostaining was performed on a Leica Biosystems BOND® RX instrument.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab228965).
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![Anti-CD8 beta antibody [EPR22331-54] - BSA and Azide free (ab254197)](https://www.abcam.com/ps/products/254/ab254197/Images/ab254197-5-benefits-of-recombinant-antibodies.png)
Anti-CD8 beta antibody [EPR22331-54] - BSA and Azide free (ab254197)
