Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-CD8 beta antibody [EPR22331-81] (ab263946)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR22331-81] to CD8 beta
  • Suitable for: Flow Cyt, IP, WB, IHC-Fr
  • Reacts with: Mouse

Overview

  • Product name

    Anti-CD8 beta antibody [EPR22331-81]
    See all CD8 beta primary antibodies

  • Description

    Rabbit monoclonal [EPR22331-81] to CD8 beta

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Mouse
    IHC-Fr
    Mouse
    IP
    Mouse
    WB
    Mouse
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Mouse thymus and lymph node lysates. Flow Cyt: Mouse thymocytes cells. IP: Mouse thymus cells. IHC-Fr: Mouse thymus tissue.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR22331-81

  • Isotype

    IgG

  • Research areas

Images

  • Western blot - Anti-CD8 beta antibody [EPR22331-81] (ab263946)
    Western blot - Anti-CD8 beta antibody [EPR22331-81] (ab263946)
    All lanes : Anti-CD8 beta antibody [EPR22331-81] (ab263946) at 1/1000 dilution

    Lane 1 : Mouse thymus lysate
    Lane 2 : Mouse lymph node lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 24 kDa
    Observed band size: 28-32 kDa
    why is the actual band size different from the predicted?



    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    CD8 beta is a glycoprotein. The molecular weight observed is consistent with what has been described in the literature (PMID: 11719190).

    Exposure time:
    Lane 1: 3 secs.
    Lane 2: 3 mins.

  • Immunohistochemistry (Frozen sections) - Anti-CD8 beta antibody [EPR22331-81] (ab263946)
    Immunohistochemistry (Frozen sections) - Anti-CD8 beta antibody [EPR22331-81] (ab263946)

    Immunohistochemical analysis of 4% PFA fixed 0.2% Triton X-100 permeabilized frozen mouse thymus tissue labeling CD8 beta with ab263946 at 1/100 (5.13 µg/ml) dilution followed by ab150077 Alexa Fluor® 488 Goat anti-Rabbit secondary at 1/1000 (2 µg/ml) dilution. The nuclear counterstain was DAPI (Blue). Positive staining on T cells of mouse thymus (PMID: 12702501) is observed.

    Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody was ab150077 Alexa Fluor® 488 Goat anti-Rabbit secondary at 1/1000 (2 µg/ml) dilution.

  • Immunohistochemistry (Frozen sections) - Anti-CD8 beta antibody [EPR22331-81] (ab263946)
    Immunohistochemistry (Frozen sections) - Anti-CD8 beta antibody [EPR22331-81] (ab263946)

    Immunohistochemical analysis of 4% PFA fixed 0.2% Triton X-100 permeabilized frozen mouse liver tissue labeling CD8 beta with ab263946 at 1/100 (5.13 µg/ml) dilution followed by ab150077 Alexa Fluor® 488 Goat anti-Rabbit secondary at 1/1000 (2 µg/ml) dilution. The nuclear counterstain was DAPI (Blue). Negative staining on mouse liver.

    Negative control: liver (PMID: 3145195).

    Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody was ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at 1/1000 (2 µg/ml) dilution.

  • Immunoprecipitation - Anti-CD8 beta antibody [EPR22331-81] (ab263946)
    Immunoprecipitation - Anti-CD8 beta antibody [EPR22331-81] (ab263946)

    CD8 beta was immunoprecipitated from 0.35 mg mouse thymus lysate 10µg with ab263946 at 1/30 dilution (2µg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab263946 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366) was used at 1/5000 dilution.

    Lane 1: Mouse thymus lysate 10µg
    Lane 2: ab263946 IP in mouse thymus lysate
    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab263946 in mouse thymus lysate

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 10 seconds.

  • Flow Cytometry - Anti-CD8 beta antibody [EPR22331-81] (ab263946)
    Flow Cytometry - Anti-CD8 beta antibody [EPR22331-81] (ab263946)

    Flow Cytometric analysis of mouse thymocytes using ab263946 at a 1/500 dilution. Cells were stained with Rabbit monoclonal IgG (ab172730) (Left) or ab263946 (Right). Then stained with anti-CD4 conjugated to Alexa Fluor® 647. Secondary used is Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at a 1/2000 dilution.

    Gated on viable cells.

  • Anti-CD8 beta antibody [EPR22331-81] (ab263946)
    Anti-CD8 beta antibody [EPR22331-81] (ab263946)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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