Antibodies, Proteins, Kits and Reagents for Life Science

321 638 ₸ Product size

100 µl 321 638 ₸ 10 µl 110 563 ₸

Order now and get it on Tuesday March 09, 2021

Anti-CD8 beta antibody [EPR22331-81] (ab263946)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR22331-81] to CD8 beta
Suitable for: Flow Cyt, IP, WB, IHC-Fr
Reacts with: Mouse

Product name

Anti-CD8 beta antibody [EPR22331-81]
See all CD8 beta primary antibodies
Description

Rabbit monoclonal [EPR22331-81] to CD8 beta
Host species

Rabbit

Tested Applications & Species

Application	Species
Flow Cyt	Mouse
IHC-Fr	Mouse
IP	Mouse
WB	Mouse

See all applications and species data

Immunogen

Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: Mouse thymus and lymph node lysates. Flow Cyt: Mouse thymocytes cells. IP: Mouse thymus cells. IHC-Fr: Mouse thymus tissue.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR22331-81
Isotype

IgG
Research areas

Western blot - Anti-CD8 beta antibody [EPR22331-81] (ab263946)

All lanes : Anti-CD8 beta antibody [EPR22331-81] (ab263946) at 1/1000 dilution

Lane 1 : Mouse thymus lysate
Lane 2 : Mouse lymph node lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 24 kDa
Observed band size: 28-32 kDa
why is the actual band size different from the predicted?

Blocking and diluting buffer and concentration: 5% NFDM/TBST.

CD8 beta is a glycoprotein. The molecular weight observed is consistent with what has been described in the literature (PMID: 11719190).

Exposure time:
Lane 1: 3 secs.
Lane 2: 3 mins.
Immunohistochemistry (Frozen sections) - Anti-CD8 beta antibody [EPR22331-81] (ab263946)

Immunohistochemical analysis of 4% PFA fixed 0.2% Triton X-100 permeabilized frozen mouse thymus tissue labeling CD8 beta with ab263946 at 1/100 (5.13 µg/ml) dilution followed by ab150077 Alexa Fluor^® 488 Goat anti-Rabbit secondary at 1/1000 (2 µg/ml) dilution. The nuclear counterstain was DAPI (Blue). Positive staining on T cells of mouse thymus (PMID: 12702501) is observed.

Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody was ab150077 Alexa Fluor^® 488 Goat anti-Rabbit secondary at 1/1000 (2 µg/ml) dilution.
Immunohistochemistry (Frozen sections) - Anti-CD8 beta antibody [EPR22331-81] (ab263946)

Immunohistochemical analysis of 4% PFA fixed 0.2% Triton X-100 permeabilized frozen mouse liver tissue labeling CD8 beta with ab263946 at 1/100 (5.13 µg/ml) dilution followed by ab150077 Alexa Fluor^® 488 Goat anti-Rabbit secondary at 1/1000 (2 µg/ml) dilution. The nuclear counterstain was DAPI (Blue). Negative staining on mouse liver.

Negative control: liver (PMID: 3145195).

Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody was ab150077 AlexaFluor^®488 Goat anti-Rabbit secondary at 1/1000 (2 µg/ml) dilution.
Immunoprecipitation - Anti-CD8 beta antibody [EPR22331-81] (ab263946)

CD8 beta was immunoprecipitated from 0.35 mg mouse thymus lysate 10µg with ab263946 at 1/30 dilution (2µg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab263946 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366) was used at 1/5000 dilution.

Lane 1: Mouse thymus lysate 10µg
Lane 2: ab263946 IP in mouse thymus lysate
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab263946 in mouse thymus lysate

Blocking and dilution buffer and concentration: 5% NFDM/TBST.

Exposure time: 10 seconds.
Flow Cytometry - Anti-CD8 beta antibody [EPR22331-81] (ab263946)

Flow Cytometric analysis of mouse thymocytes using ab263946 at a 1/500 dilution. Cells were stained with Rabbit monoclonal IgG (ab172730) (Left) or ab263946 (Right). Then stained with anti-CD4 conjugated to Alexa Fluor^® 647. Secondary used is Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077) at a 1/2000 dilution.

Gated on viable cells.
Anti-CD8 beta antibody [EPR22331-81] (ab263946)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com