Antibodies, Proteins, Kits and Reagents for Life Science

291 484 ₸ Product size

100 µl 291 484 ₸

Order now and get it on Wednesday March 03, 2021

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR5857] to RCC1
Suitable for: Flow Cyt, WB, IHC-P
Reacts with: Human

Product name

Anti-RCC1 antibody [EPR5857]
See all RCC1 primary antibodies
Description

Rabbit monoclonal [EPR5857] to RCC1
Host species

Rabbit

Tested Applications & Species

Application	Species
Flow Cyt	Human
IHC-P	Human
WB	Human

See all applications and species data

Immunogen

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: A431, HeLa, Jurkat and 293T cell lysates. IHC-P: Human bladder carcinoma tissue. Flow Cyt: HeLa cells.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C.
Storage buffer

pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant
Concentration information loading...
Purity

Tissue culture supernatant
Clonality

Monoclonal
Clone number

EPR5857
Isotype

IgG
Research areas

Western blot - Anti-RCC1 antibody [EPR5857] (ab109379)

All lanes : Anti-RCC1 antibody [EPR5857] (ab109379) at 1/1000 dilution

Lane 1 : A431 cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : Jurkat cell lysate
Lane 4 : 293T cell lysate

Lysates/proteins at 10 µg per lane.

Predicted band size: 45 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RCC1 antibody [EPR5857] (ab109379)

ab109379, at a 1/100 dilution, staining RCC1 in paraffin-embedded Human bladder carcinoma tissue

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Flow Cytometry - Anti-RCC1 antibody [EPR5857] (ab109379)

Flow cytometry analysis of HeLa (human cervix adenocarcinoma) cells labeling RCC1 (red) with ab109379 at a 1/2000 dilution. Cells were fixed with 4% paraformaldehyde and permeabilized with 90% methanol. A goat anti-rabbit IgG (Alexa Fluor^® 488) (ab150077) was used as the secondary antibody at a 1/2000 dilution. Black - Rabbit monoclonal IgG (ab172730). Blue (unlabeled control) - Cells without incubation with primary and secondary antibodies.
Anti-RCC1 antibody [EPR5857] (ab109379)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com