Anti-RCC1 antibody (ab152156)
Key features and details
- Rabbit polyclonal to RCC1
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-RCC1 antibody
See all RCC1 primary antibodies -
Description
Rabbit polyclonal to RCC1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Recombinant fragment corresponding to a region within amino acids 2-421 of Human RCC1 (Uniprot ID P18754).
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Positive control
- Human 293T, A431, H1299, HeLa S3, HepG2, MOLT4 and Raji whole cell lysates; mouse Neuro2A and GL261 whole cell lysates/extracts; A431 cells; Human KYSE170 xenograft tissue.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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All lanes : Anti-RCC1 antibody (ab152156) at 1/1000 dilution
Lane 1 : 293T whole cell lysate
Lane 2 : A431 whole cell lysate
Lane 3 : H1299 whole cell lysate
Lane 4 : HeLa S3 whole cell lysate
Lane 5 : HepG2 whole cell lysate
Lane 6 : MOLT4 whole cell lysate
Lane 7 : Raji whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 45 kDa
10% SDS PAGE -
Immunofluorescence analysis of paraformaldehyde-fixed A431 cells, staining RCC1 (green) using ab152156 at a 1/200 dilution.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RCC1 antibody (ab152156)Immunohistochemical analysis of paraffin-embedded Human KYSE170 xenograft tissue, staining RCC1 using ab152156 at a 1/500 dilution.
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All lanes : Anti-RCC1 antibody (ab152156) at 1/1000 dilution
Lane 1 : Neuro2A whole cell lysate/extract
Lane 2 : GL261 whole cell lysate/extract
Lysates/proteins at 30 µg per lane.
Predicted band size: 45 kDa
12% SDS PAGE