ChIP Kit (Magnetic, qPCR) (ab270816)
Overview
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Product name
ChIP Kit (Magnetic, qPCR) -
Product overview
ChIP Kit (Magnetic, qPCR) (ab270816) is a highly optimized and validated solution for ChIP-qPCR assays for either histones or transcription factors. The complete kit contains everything you need for start-to-finish ChIP including all validated buffers and reagents for chromatin shearing, immunoprecipitation, and DNA isolation for exceptional ChIP-qPCR results.
This kit uses a unique and fast method for DNA isolation and decrosslinking within 30 minutes compared to the standard 4 hours. Overall, easy to use and rapid protocol allows to receive the results within 20 hours with 4 hours hands-on time.
This kit contains enough reagents to perform 4 chromatin preparations, 24 chromatin immunoprecipitations and 32 DNA isolations.
Sample size:
Histones -
Cells (100,000 cells to 1,000,000 cells per IP)
Tissues (1.5 mg to 5 mg of tissue per IP)
Transciption factors -
Cells (4,000,000 cells per IP)
Tissues (30 mg of tissue per IP)
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Notes
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Properties
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Storage instructions
Please refer to protocols. -
Components 24 tests 5% BSA (DNA free) 1 x 784µl 5x ChIP Buffer C1b 1 x 6.9ml ChIP-seq grade water 1 x 26.6ml Protein A-coated magnetic beads 1 x 720µl DNA isolation buffer (DIB) 1 x 3.2ml Elution Buffer E1 1 x 220µl Elution Buffer E2 1 x 20µl Fixation buffer 1 x 8ml Glycine 1 x 8.8ml Lysis Buffer L1b 1 x 100ml Lysis Buffer L2 1 x 60ml Protease inhibitor cocktail 1 x 80µl Proteinase K 1 x 32µl Rabbit IgG 1 x 8µg Shearing Buffer S1b 1 x 9ml Wash buffer W1 1 x 8.4ml Wash buffer W2 1 x 8.4ml Wash buffer W3 1 x 8.4ml Wash buffer W4 1 x 8.4ml
