Anti-PNPLA3 antibody (ab81874)
Key features and details
- Rabbit polyclonal to PNPLA3
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-PNPLA3 antibody
See all PNPLA3 primary antibodies -
Description
Rabbit polyclonal to PNPLA3 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Human -
Immunogen
Synthetic peptide corresponding to Human PNPLA3 aa 150-250 conjugated to keyhole limpet haemocyanin.
(Peptide available asab102097) -
Positive control
- This antibody gave a positive signal in the following lysates: Human Liver Tissue; HepG2 Whole Cell Lysate.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentrationConcentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Immunizing Peptide (Blocking)
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab81874 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
GuaranteedTested applications are guaranteed to work and covered by our Abpromise guarantee.
PredictedPredicted to work for this combination of applications and species but not guaranteed.
IncompatibleDoes not work for this combination of applications and species.
Application Species WB HumanAll applications MouseApplication Abreviews Notes WB (1) Use a concentration of 1 µg/ml. Detects a band of approximately 55 kDa (predicted molecular weight: 53 kDa).Notes WB
Use a concentration of 1 µg/ml. Detects a band of approximately 55 kDa (predicted molecular weight: 53 kDa).Target
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Relevance
PNPLA3 is a multifunctional enzyme which has both triacylglycerol lipase and acylglycerol O-acyltransferase activities. It contains one patatin domain. -
Cellular localization
Membrane; Single pass type II membrane protein -
Database links
- Entrez Gene: 80339 Human
- Entrez Gene: 116939 Mouse
- Omim: 609567 Human
- SwissProt: Q9NST1 Human
- SwissProt: Q91WW7 Mouse
- Unigene: 654800 Human
- Unigene: 390817 Mouse
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Alternative names
- Acylglycerol O acyltransferase antibody
- Adiponutrin antibody
- ADPN antibody
see all
Images
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All lanes : Anti-PNPLA3 antibody (ab81874) at 1 µg/ml
Lane 1 : Human liver tissue lysate
Lane 2 : HepG2 whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/50000 dilution
Predicted band size: 53 kDa
Observed band size: 35,55,70 kDa why is the actual band size different from the predicted?Blocking buffer: 2% BSA
Gel type: MOPS
Exposure Time: 12 minutes
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All lanes : ab81874 at 1 µg/ml
Lane 1 : Human liver
Lane 2 : Human whole cell lysate HepG2
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : ab97080 at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 53 kDa
Observed band size: 52 kDa why is the actual band size different from the predicted?
Additional bands at: 120 kDa, 48 kDa. We are unsure as to the identity of these extra bands.The PNPLA3 sequence contains two glycosylation sites (Swissprot data). The additional band close to the band of interest might be a glycosylated form of PNPLA3. Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented above.
Protocols
Datasheets and documents
References (9)
ab81874 has been referenced in 9 publications.
- Yuan S et al. PNPLA3 I148M mediates the regulatory effect of NF-kB on inflammation in PA-treated HepG2 cells. J Cell Mol Med 24:1541-1552 (2020). PubMed: 31793207
- Wagner C et al. Lysosomal acid lipase is the major acid retinyl ester hydrolase in cultured human hepatic stellate cells but not essential for retinyl ester degradation. Biochim Biophys Acta Mol Cell Biol Lipids 1865:158730 (2020). PubMed: 32361002
- Liu Y et al. Effects of daphnetin on lipid metabolism, insulin resistance and oxidative stress in OA-treated HepG2 cells. Mol Med Rep 19:4673-4684 (2019). PubMed: 30957185
- Yang A et al. Dynamic interactions of ABHD5 with PNPLA3 regulate triacylglycerol metabolism in brown adipocytes. Nat Metab 1:560-569 (2019). PubMed: 31497752
- Negoita F et al. PNPLA3 variant M148 causes resistance to starvation-mediated lipid droplet autophagy in human hepatocytes. J Cell Biochem 120:343-356 (2019). PubMed: 30171718
- Liu Z et al. Overexpression of variant PNPLA3 gene at I148M position causes malignant transformation of hepatocytes via IL-6-JAK2/STAT3 pathway in low dose free fatty acid exposure: a laboratory investigation in vitro and in vivo. Am J Transl Res 8:1319-38 (2016). Human . PubMed: 27186262
- McCann CC et al. Hepatic patatin-like phospholipase domain-containing protein 3 sequence, single nucleotide polymorphism presence, protein confirmation, and responsiveness to energy balance in dairy cows. J Dairy Sci 97:5167-75 (2014). PubMed: 24931521
- Xu F et al. SIRT1 mediates the effect of GLP-1 receptor agonist exenatide on ameliorating hepatic steatosis. Diabetes 63:3637-46 (2014). WB ; Mouse . PubMed: 24947350
- Hao L et al. Shifts in dietary carbohydrate-lipid exposure regulate expression of the non-alcoholic fatty liver disease-associated gene PNPLA3/adiponutrin in mouse liver and HepG2 human liver cells. Metabolism 63:1352-62 (2014). WB ; Mouse . PubMed: 25060692
Images
-
All lanes : Anti-PNPLA3 antibody (ab81874) at 1 µg/ml
Lane 1 : Human liver tissue lysate
Lane 2 : HepG2 whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/50000 dilution
Predicted band size: 53 kDa
Observed band size: 35,55,70 kDa why is the actual band size different from the predicted?Blocking buffer: 2% BSA
Gel type: MOPS
Exposure Time: 12 minutes
-
All lanes : ab81874 at 1 µg/ml
Lane 1 : Human liver
Lane 2 : Human whole cell lysate HepG2
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : ab97080 at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 53 kDa
Observed band size: 52 kDa why is the actual band size different from the predicted?
Additional bands at: 120 kDa, 48 kDa. We are unsure as to the identity of these extra bands.The PNPLA3 sequence contains two glycosylation sites (Swissprot data). The additional band close to the band of interest might be a glycosylated form of PNPLA3. Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented above.