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Anti-PNPLA3 antibody (ab81874)

Price and availability

301 536 ₸

Availability

Order now and get it on Thursday February 25, 2021

Anti-PNPLA3 antibody (ab81874)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Rabbit polyclonal to PNPLA3
  • Suitable for: WB
  • Reacts with: Human
  • Isotype: IgG

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Overview

  • Product name

    Anti-PNPLA3 antibody
    See all PNPLA3 primary antibodies
  • Description

    Rabbit polyclonal to PNPLA3
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    WB
    Human
    See all applications and species data
  • Immunogen

    Synthetic peptide corresponding to Human PNPLA3 aa 150-250 conjugated to keyhole limpet haemocyanin.
    (Peptide available as ab102097)

  • Positive control

    • This antibody gave a positive signal in the following lysates: Human Liver Tissue; HepG2 Whole Cell Lysate.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituent: PBS

    Batches of this product that have a concentration
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Cardiovascular
    • Lipids / Lipoproteins
    • Lipid Metabolism
    • Lipases
    • Signal Transduction
    • Metabolism
    • Energy Metabolism
    • Signal Transduction
    • Metabolism
    • Lipid metabolism
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Lipid and lipoprotein metabolism
    • Lipases
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Energy transfer pathways
    • Energy Metabolism
    • Metabolism
    • Types of disease
    • Cancer

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Immunizing Peptide (Blocking)

    • Human PNPLA3 peptide (ab102097)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Recombinant Protein

    • Recombinant Human PNPLA3 protein (ab132787)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab81874 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
WB
Human
All applications
Mouse
Application Abreviews Notes
WB (1)
Use a concentration of 1 µg/ml. Detects a band of approximately 55 kDa (predicted molecular weight: 53 kDa).
Notes
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 55 kDa (predicted molecular weight: 53 kDa).

Target

  • Relevance

    PNPLA3 is a multifunctional enzyme which has both triacylglycerol lipase and acylglycerol O-acyltransferase activities. It contains one patatin domain.
  • Cellular localization

    Membrane; Single pass type II membrane protein
  • Database links

    • Entrez Gene: 80339 Human
    • Entrez Gene: 116939 Mouse
    • Omim: 609567 Human
    • SwissProt: Q9NST1 Human
    • SwissProt: Q91WW7 Mouse
    • Unigene: 654800 Human
    • Unigene: 390817 Mouse
    • Alternative names

      • Acylglycerol O acyltransferase antibody
      • Adiponutrin antibody
      • ADPN antibody
      • C22orf20 antibody
      • Calcium independent phospholipase A2 epsilon antibody
      • DJ796I17.1 antibody
      • iPLA(2)epsilon antibody
      • IPLA2-epsilon antibody
      • Patatin like phospholipase domain containing 3 antibody
      • patatin like phospholipase domain containing protein 3 antibody
      • phospholipase A2, calcium independent, epsilon antibody
      see all

    Images

    • Western blot - Anti-PNPLA3 antibody (ab81874)
      Western blot - Anti-PNPLA3 antibody (ab81874)
      All lanes : Anti-PNPLA3 antibody (ab81874) at 1 µg/ml

      Lane 1 : Human liver tissue lysate
      Lane 2 : HepG2 whole cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/50000 dilution

      Predicted band size: 53 kDa
      Observed band size: 35,55,70 kDa
      why is the actual band size different from the predicted?



      Blocking buffer: 2% BSA

      Gel type: MOPS

      Exposure Time: 12 minutes

    • Western blot - Anti-PNPLA3 antibody (ab81874)
      Western blot - Anti-PNPLA3 antibody (ab81874)
      All lanes : ab81874 at 1 µg/ml

      Lane 1 : Human liver
      Lane 2 : Human whole cell lysate HepG2

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : ab97080 at 1/5000 dilution

      Developed using the ECL technique.

      Performed under reducing conditions.

      Predicted band size: 53 kDa
      Observed band size: 52 kDa why is the actual band size different from the predicted?
      Additional bands at: 120 kDa, 48 kDa. We are unsure as to the identity of these extra bands.



      The PNPLA3 sequence contains two glycosylation sites (Swissprot data). The additional band close to the band of interest might be a glycosylated form of PNPLA3. Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented above.

    Protocols

    • Western blot protocols
    • Immunocytochemistry & immunofluorescence protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
  • References (9)

    Publishing research using ab81874? Please let us know so that we can cite the reference in this datasheet.

    ab81874 has been referenced in 9 publications.

    • Yuan S  et al. PNPLA3 I148M mediates the regulatory effect of NF-kB on inflammation in PA-treated HepG2 cells. J Cell Mol Med 24:1541-1552 (2020). PubMed: 31793207
    • Wagner C  et al. Lysosomal acid lipase is the major acid retinyl ester hydrolase in cultured human hepatic stellate cells but not essential for retinyl ester degradation. Biochim Biophys Acta Mol Cell Biol Lipids 1865:158730 (2020). PubMed: 32361002
    • Liu Y  et al. Effects of daphnetin on lipid metabolism, insulin resistance and oxidative stress in OA-treated HepG2 cells. Mol Med Rep 19:4673-4684 (2019). PubMed: 30957185
    • Yang A  et al. Dynamic interactions of ABHD5 with PNPLA3 regulate triacylglycerol metabolism in brown adipocytes. Nat Metab 1:560-569 (2019). PubMed: 31497752
    • Negoita F  et al. PNPLA3 variant M148 causes resistance to starvation-mediated lipid droplet autophagy in human hepatocytes. J Cell Biochem 120:343-356 (2019). PubMed: 30171718
    • Liu Z  et al. Overexpression of variant PNPLA3 gene at I148M position causes malignant transformation of hepatocytes via IL-6-JAK2/STAT3 pathway in low dose free fatty acid exposure: a laboratory investigation in vitro and in vivo. Am J Transl Res 8:1319-38 (2016). Human . PubMed: 27186262
    • McCann CC  et al. Hepatic patatin-like phospholipase domain-containing protein 3 sequence, single nucleotide polymorphism presence, protein confirmation, and responsiveness to energy balance in dairy cows. J Dairy Sci 97:5167-75 (2014). PubMed: 24931521
    • Xu F  et al. SIRT1 mediates the effect of GLP-1 receptor agonist exenatide on ameliorating hepatic steatosis. Diabetes 63:3637-46 (2014). WB ; Mouse . PubMed: 24947350
    • Hao L  et al. Shifts in dietary carbohydrate-lipid exposure regulate expression of the non-alcoholic fatty liver disease-associated gene PNPLA3/adiponutrin in mouse liver and HepG2 human liver cells. Metabolism 63:1352-62 (2014). WB ; Mouse . PubMed: 25060692

    Images

    • Western blot - Anti-PNPLA3 antibody (ab81874)
      Western blot - Anti-PNPLA3 antibody (ab81874)
      All lanes : Anti-PNPLA3 antibody (ab81874) at 1 µg/ml

      Lane 1 : Human liver tissue lysate
      Lane 2 : HepG2 whole cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/50000 dilution

      Predicted band size: 53 kDa
      Observed band size: 35,55,70 kDa
      why is the actual band size different from the predicted?



      Blocking buffer: 2% BSA

      Gel type: MOPS

      Exposure Time: 12 minutes

    • Western blot - Anti-PNPLA3 antibody (ab81874)
      Western blot - Anti-PNPLA3 antibody (ab81874)
      All lanes : ab81874 at 1 µg/ml

      Lane 1 : Human liver
      Lane 2 : Human whole cell lysate HepG2

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : ab97080 at 1/5000 dilution

      Developed using the ECL technique.

      Performed under reducing conditions.

      Predicted band size: 53 kDa
      Observed band size: 52 kDa why is the actual band size different from the predicted?
      Additional bands at: 120 kDa, 48 kDa. We are unsure as to the identity of these extra bands.



      The PNPLA3 sequence contains two glycosylation sites (Swissprot data). The additional band close to the band of interest might be a glycosylated form of PNPLA3. Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented above.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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