Anti-RCC1 antibody (ab70749)
Key features and details
- Goat polyclonal to RCC1
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-RCC1 antibody
See all RCC1 primary antibodies -
Description
Goat polyclonal to RCC1 -
Host species
Goat -
Tested Applications & Species
See all applications and species dataApplication Species IP HumanWB Human
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Immunogen
Synthetic peptide corresponding to a region between residue 1 and 50 of human RCC1 (NP_001260.1).
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Positive control
- WB: Whole cell lysate from HeLa or 293T cells; IP: HeLa whole cell lysate.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituents: 1.815% Tris, 1.764% Sodium citrate, 0.021% PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunoprecipitation - Anti-RCC1 antibody (ab70749)
Detection of human RCC1 by western blot of immunoprecipitates.
Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer.
Lane 1: Anti-RCC1 antibody ab70749 used for IP at 6 µg per reaction
Lane 2: Anti-RCC1 antibody, ab245594
Lane 3: IgG Control
For blotting immunoprecipitated RCC1, ab70749 was used at 1 µg/mL.
Detection: Chemiluminescence with an exposure time of 30 seconds.
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Western blot - Anti-RCC1 antibody (ab70749)All lanes : Anti-RCC1 antibody (ab70749) at 1/10000 dilution
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : 293T whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 45 kDa
Observed band size: 45 kDa
Additional bands at: 28 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 3 minutes
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Western blot - Anti-RCC1 antibody (ab70749)All lanes : Anti-RCC1 antibody (ab70749) at 1/2000 dilution
Lane 1 :Recombinant Human RCC1 protein (ab90625) at 0.01 µg
Lane 2 :Recombinant Human RCC1 protein (ab90625) at 0.1 µg
Secondary
All lanes : Rabbit polyclonal to Goat IgG - H&L - Pre-Adsorbed (HRP) (ab65486) at 1/3000 dilution (Rabbit polyclonal to Goat IgG - H&L - Pre-Adsorbed (HRP))
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 45 kDa
Exposure time: 8 minutes
