Rat heart tissue lysate - mitochondrial extract (ab110347)
Overview
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Product name
Rat heart tissue lysate - mitochondrial extract
See all Rat Heart lysates -
Description
Rat Heart Mitochondrial lysate -
General notes
Product was previously marketed under the MitoSciences sub-brand.
2 mg of rat heart mitochondria in 400ul of iso-osmotic buffer. This sample can act as a positive control in immunocapture applications and Blue Native PAGE. Immunocapture, however could also be used for electrophoresis and Western blotting, and Enzyme Isolations. 1 mg solubilized mitochondria for Immunocapture experiments. For the Immunocapture of OXPHOS complexes it is recommended that 180 µl of the mitochondrial membranes supplied be solubilized by the addition of 20 µl of 10% lauryl maltoside. Insoluble material is removed by centrifugation at 72,000g before immunocapture of complexes from the supernatant.
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Tested applications
Suitable for: IP, SDS-PAGE, WBmore details
Properties
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Mycoplasma free
Yes -
Form
Liquid -
Storage instructions
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles. -
Storage buffer
Constituents: 0.02% PMSF, 0.16% Tris, 0.89% EDTA, 8.56% Sucrose -
Concentration information loading... -
Lysate notes
2 mg of rat heart mitochondria in 400ul of iso-osmotic buffer. This sample can act as a positive control in immunocapture applications and Blue Native PAGE. Immunocapture, however could also be used for electrophoresis and Western blotting, and Enzyme Isolations. 1 mg solubilized mitochondria for Immunocapture experiments. For the Immunocapture of OXPHOS complexes it is recommended that 180 µl of the mitochondrial membranes supplied be solubilized by the addition of 20 µl of 10% lauryl maltoside. Insoluble material is removed by centrifugation at 72,000g before immunocapture of complexes from the supernatant.
