Anti-MAK16 antibody (ab185101)
Key features and details
- Rabbit polyclonal to MAK16
- Suitable for: ICC/IF, WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-MAK16 antibody -
Description
Rabbit polyclonal to MAK16 -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, WB, IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Cow, Xenopus laevis, Zebrafish, Xenopus tropicalis
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Immunogen
Recombinant fragment corresponding to Human MAK16 aa 100-200 (internal sequence).
Database link: Q9BXY0 -
Positive control
- IHC-P: Human cerebellum tissue WB: Transfected HEK-293T whole cell lysate; ICC: A-431 whole cells.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 59% PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAK16 antibody (ab185101)
Immunohistochemical analysis of paraffin-embedded human cerebellum tissue labeling MAK16 in the nucleolus of Purkinje cells using ab185101 at a 1/2500 dilution.
Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Western blot - Anti-MAK16 antibody (ab185101)All lanes : Anti-MAK16 antibody (ab185101) at 0.4 µg/ml
Lane 1 : Control (vector only transfected) - HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 2 : MAK16 over-expression - HEK-293T whole cell lysate, (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa)
Predicted band size: 35 kDa
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Immunocytochemistry/ Immunofluorescence - Anti-MAK16 antibody (ab185101)Immunocytochemistry analysis of A431 (human epidermoid carcinoma cell line) whole cells labeling MAK16 in the nucleolus of Purkinje cells using ab185101 at 2 µg/ml.