Mouse Progranulin Matched Antibody Pair Kit (ab219524)
Key features and details
- Unlabeled capture antibody, biotin-labeled detection antibody and calibrated protein standard
- For economical ELISA and ELISA-based assay development
- Reacts with: Mouse
- Range: 15.6 pg/ml - 1000 pg/ml
Overview
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Product name
Mouse Progranulin Matched Antibody Pair Kit
See all Granulin kits -
Detection method
Colorimetric -
Assay type
ELISA set -
Sensitivity
5.16 pg/ml -
Range
15.6 pg/ml - 1000 pg/ml -
Species reactivity
Reacts with: Mouse -
Product overview
Mouse Progranulin Matched Antibody Pair Kits include a capture and a biotinylated detector antibody pair, along with a calibrated protein standard, suitable for sandwich ELISA. The Matched Antibody Pair Kit can be used to quantify native and recombinant mouse Progranulin.
Optimization of the kit reagents to sample type, immunoassay format or instrumentation may be required. Guidelines for use of this kit in a standard 96-well microplate sandwich ELISA using HRP/TMB system of colorimetric detection is described in this assay procedure for the purposes of quantification.
Protocol information and tips on the use of the Matched Antibody Pair kits for sandwich ELISA can be found on our website. An accessory pack can be purchased which includes buffer reagents required to perform 10 x 96-well plate sandwich ELISAs (ab210905).
For additional information on the performance of the antibody pair used in this kit, please see our equivalent SimpleStep ELISA kit ab213473. Please note that while the antibody pair is the same provided in the corresponding SimpleStep ELISA Kit, due to differences in their formulation, this antibody pair cannot be used with the consumables provided with our SimpleStep ELISA Kits.
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Tested applications
Suitable for: ELISAmore details -
Platform
Reagents
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 10 x 96 tests 5 x 96 tests Mouse Progranulin Capture Antibody 2 x 50µg 1 x 50µg Mouse Progranulin Detector Antibody 2 x 12.5µg 1 x 12.5µg Mouse Progranulin Lyophilized Protein 2 vials 1 vial -
Research areas
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Function
Granulins have possible cytokine-like activity. They may play a role in inflammation, wound repair, and tissue remodeling.
Granulin-4 promotes proliferation of the epithelial cell line A431 in culture while granulin-3 acts as an antagonist to granulin-4, inhibiting the growth. -
Tissue specificity
In myelogenous leukemic cell lines of promonocytic, promyelocytic, and proerythroid lineage, in fibroblasts, and very strongly in epithelial cell lines. Present in inflammatory cells and bone marrow. Highest levels in kidney. -
Involvement in disease
Defects in GRN are the cause of ubiquitin-positive frontotemporal dementia (UP-FTD) [MIM:607485]; also known as tau-negative frontotemporal dementia linked to chromosome 17. Frontotemporal dementia (FTD) is the second most common cause of dementia in people under the age of 65 years. It is an autosomal dominant neurodegenerative disease. -
Sequence similarities
Belongs to the granulin family. -
Post-translational
modificationsGranulins are disulfide bridged. -
Cellular localization
Secreted. - Information by UniProt
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Alternative names
- Acrogranin
- CLN11
- GEP
see all -
Database links
- Entrez Gene: 14824 Mouse
- SwissProt: P28798 Mouse
- Unigene: 1568 Mouse
Images
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Mouse Progranulin standard curve
Standard calibration curve. Background subtracted values are graphed.
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Sandwich ELISA - Mouse Progranulin Matched Antibody Pair Kit (ab219524)To learn more about the advantages of recombinant antibodies see here.
