Anti-RPB3 antibody (ab138436)
Key features and details
- Rabbit polyclonal to RPB3
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-RPB3 antibody
See all RPB3 primary antibodies -
Description
Rabbit polyclonal to RPB3 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IP HumanWB Human
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Immunogen
Synthetic peptide corresponding to Human RPB3 aa 225-275.
Sequence:KPERFY YNVESCGSLR PETIVLSALS GLKKKLSDLQ TQLSHEIQSD VLTIN
Database link: NP_116558.1 -
Positive control
- HeLa, Jurkat and 293T cell lysates.
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General notes
Previously labelled as POLR2C.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituent: 99.01% Tris citrate/phosphate
pH 7-8 -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-RPB3 antibody (ab138436)All lanes : Anti-RPB3 antibody (ab138436) at 1/2000 dilution
Lane 1 : HeLa cell lysate at 50 µg
Lane 2 : HeLa cell lysate at 15 µg
Lane 3 : 293T cell lysate at 50 µg
Lane 4 : Jurkat cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 31 kDa
Exposure time: 3 minutes
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Immunoprecipitation - Anti-RPB3 antibody (ab138436)Immunoprecipitation detection of RPB3 in HeLa whole cell lysate (1 mg/IP; 20% of IP loaded/lane) using ab138436 at 6µg/mg lysate. Subsequent Western blot detection step was performed using ab138436 at 1µg/ml. Chemiluminescence with an exposure time of 10 seconds.
