Anti-RPB3 antibody - C-terminal (ab230893)
Key features and details
- Rabbit polyclonal to RPB3 - C-terminal
- Suitable for: WB, ICC/IF, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-RPB3 antibody - C-terminal
See all RPB3 primary antibodies -
Description
Rabbit polyclonal to RPB3 - C-terminal -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IF, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Cow
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Immunogen
Synthetic peptide corresponding to Human RPB3 aa 200 to the C-terminus (C terminal) conjugated to keyhole limpet haemocyanin.
Database link: P19387 -
Positive control
- IHC-P: Human cerebellum tissue. ICC/IF: HeLa cells. WB: Hela and U-251 MG cell lysate; mouse skeletal muscle tissue lysate.
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General notes
Previously labelled as POLR2C.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-RPB3 antibody - C-terminal (ab230893)All lanes : Anti-RPB3 antibody - C-terminal (ab230893) at 1/1000 dilution
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate
Lane 2 : U-251 MG (human brain glioma cell line) cell lysate
Lane 3 : Mouse skeletal muscle tissue lysate
Lysates/proteins at 35 µg per lane.
Developed using the ECL technique.
Predicted band size: 31 kDa
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Immunocytochemistry/ Immunofluorescence - Anti-RPB3 antibody - C-terminal (ab230893)HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for RPB3 using ab230893 at a dilution of 1/25 in ICC/IF. An Alexa Fluor® 488-conjugated goat anti-rabbit lgG at 1/400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Alexa Fluor® 555 conjugated with Phalloidin (red).
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPB3 antibody - C-terminal (ab230893)Formalin-fixed, paraffin-embedded human cerebellum tissue stained for RPB3 with ab230893 at a 1/100 in immunohistochemical analysis.
