Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Fyn antibody [EPR5500] (ab125016)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR5500] to Fyn
  • Suitable for: WB, ICC/IF
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-Fyn antibody [EPR5500]
    See all Fyn primary antibodies

  • Description

    Rabbit monoclonal [EPR5500] to Fyn

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    ICC/IF
    Mouse
    WB
    Mouse
    Rat
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide within Human Fyn aa 1-100. The exact sequence is proprietary.
    Database link: P06241

  • Positive control

    • WB: Ramos, Jurkat, HEK-293 and NIH/3T3 cell lysates. Mouse and rat brain tissue lysate. ICC/IF: Neuro-2a cells.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

Images

  • Western blot - Anti-Fyn antibody [EPR5500] (ab125016)
    Western blot - Anti-Fyn antibody [EPR5500] (ab125016)
    All lanes : Anti-Fyn antibody [EPR5500] (ab125016) at 1/1000 dilution (purified)

    Lane 1 : Jurkat (Human T cell leukemia cell line from peripheral blood) cell lysate
    Lane 2 : Ramos (Human Burkitt's lymphoma cell line) cell lysate
    Lane 3 : HEK-293 (Human epithelial cell line from embryonic kidney) cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : HRP goat anti-rabbit (H+L) at 1/1000 dilution

    Predicted band size: 60 kDa
    Observed band size: 60 kDa



    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Immunocytochemistry/ Immunofluorescence - Anti-Fyn antibody [EPR5500] (ab125016)
    Immunocytochemistry/ Immunofluorescence - Anti-Fyn antibody [EPR5500] (ab125016)

    Immunofluorescence staining of Neuro-2a (Mouse neuroblastoma cell line) cells with purified ab125016 at a working dilution of 1 in 500, counter-stained with DAPI. The secondary antibody was Alexa Fluor® 488 goat anti rabbit (ab150077), used at a dilution of 1 in 500. ab7291 was used to stain tubulin, and this is shown in the top right hand panel. The cells were fixed in 4% PFA and permeabilized using 0.1% Triton X 100.

    The negative control is shown in bottom middle and right hand panels - for the negative controls, purified ab125016 was used at a dilution of 1/200 followed by an Alexa Fluor® 594 goat anti-mouse antibody at a dilution of 1/500.

  • Western blot - Anti-Fyn antibody [EPR5500] (ab125016)
    Western blot - Anti-Fyn antibody [EPR5500] (ab125016)
    All lanes : Anti-Fyn antibody [EPR5500] (ab125016) at 1/5000 dilution (purified)

    Lane 1 : Mouse brain tissue lysate
    Lane 2 : Rat brain tissue lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : HRP goat anti-rabbit (H+L) at 1/1000 dilution

    Predicted band size: 60 kDa
    Observed band size: 60 kDa



    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-Fyn antibody [EPR5500] (ab125016)
    Western blot - Anti-Fyn antibody [EPR5500] (ab125016)
    All lanes : Anti-Fyn antibody [EPR5500] (ab125016) at 1/1000 dilution (unpurified)

    Lane 1 : Ramos (Human Burkitt's lymphoma cell line) lysate
    Lane 2 : Jurkat (Human T cell leukemia cell line from peripheral blood) lysate
    Lane 3 : NIH/3T3 (Mouse embryo fibroblast cell line) lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat anti-Rabbit HRP at 1/2000 dilution

    Predicted band size: 60 kDa
    Observed band size: 59 kDa
    why is the actual band size different from the predicted?

  • Anti-Fyn antibody [EPR5500] (ab125016)
    Anti-Fyn antibody [EPR5500] (ab125016)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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