Anti-Fyn antibody [2A10] (ab119855)
Key features and details
- Mouse monoclonal [2A10] to Fyn
- Suitable for: WB, ELISA, IHC-P, Flow Cyt, ICC/IF
- Reacts with: Human, Recombinant fragment
- Isotype: IgG1
Overview
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Product name
Anti-Fyn antibody [2A10]
See all Fyn primary antibodies -
Description
Mouse monoclonal [2A10] to Fyn -
Host species
Mouse -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt HumanICC/IF HumanIHC-P HumanWB Recombinant fragment -
Immunogen
Purified recombinant fragment of Human Fyn, expressed in E. coli.
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Positive control
- IHC-P: Human breast cancer and brain tissues ICC/IF: U251 cells Flow Cyt: Hela cells
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituent: PBS -
Concentration information loading...
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Purity
Protein G purified -
Purification notes
Purified from tissue culture supernatant. -
Clonality
Monoclonal -
Clone number
2A10 -
Isotype
IgG1 -
Research areas
Images
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Anti-Fyn antibody [2A10] (ab119855) at 1/500 dilution + Fyn recombinant protein (7-176 amino acids)
Predicted band size: 61 kDa
Predicted MW of Recombinant Fragment : 44.3 kDa -
ab119855, at a 1/200 dilution, staining Fyn in paraffin embedded Human breast cancer tissue by Immunohistochemistry, with DAB staining.
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ab119855, at a 1/200 dilution, staining Fyn in paraffin embedded Human brain tissue by Immunohistochemistry, with DAB staining.
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Immunofluorescence analysis of U251 cells, using ab119855 at a 1/200 dilution (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
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Flow Cytometric analysis of Hela cells, using ab119855 at a 1/200 dilution (green) and negative control (purple).
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Elisa results for ab119855