Immunocytochemistry analysis of Fyn in murine transformed fibroblasts using ab1881 (red) at 5 µL. Actin cytoskeleton was decorated by phalloidin (green) and cell nuclei stained with DAPI (blue).

Overlay histogram showing Ramos cells stained with ab1881 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab1881, 1µg/1x10⁶ cells) for 30 min at 22ºC. The secondary antibody used was DyLight^® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 2µg/1x10⁶ cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in Ramos cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

Immunocytochemistry analysis of Fyn in human HeLa cell line using ab1881 (green) at 5 µL. Actin cytoskeleton was decorated by phalloidin (red) and cell nuclei stained with DAPI (blue).