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Signal Transduction Signaling Pathway G Protein Signaling Small G Proteins Other

Anti-Fyn antibody (ab76934)

Anti-Fyn antibody (ab76934)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Mouse monoclonal to Fyn
  • Suitable for: WB
  • Reacts with: Human
  • Isotype: IgG1

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Overview

  • Product name

    Anti-Fyn antibody
    See all Fyn primary antibodies
  • Description

    Mouse monoclonal to Fyn
  • Host species

    Mouse
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant full length protein, corresponding to amino acids 1-483 of Human Fyn (AAH32496), with a 26kDa enzyme tag.

  • Positive control

    • FYN transfected 293T cell lysate
  • General notes

    This product was changed from ascites to tissue culture supernatant on 30th April 2019. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer

    pH: 7.4
    Constituent: PBS
  • Concentration information loading...
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Isotype

    IgG1
  • Light chain type

    kappa
  • Research areas

    • Neuroscience
    • Neurology process
    • Neurodegenerative disease
    • Alzheimer's disease
    • Other
    • Signal Transduction
    • Protein Phosphorylation
    • Tyrosine Kinases
    • Src Family
    • Cancer
    • Oncoproteins/suppressors
    • Oncoproteins
    • Signal transducers

Images

  • Western blot - Anti-Fyn antibody (ab76934)
    Western blot - Anti-Fyn antibody (ab76934)
    Anti-Fyn antibody (ab76934) at 5 µg/ml + Immunogen at 0.2 µg

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/5000 dilution

    Predicted band size: 60 kDa
    Observed band size: 86 kDa
    why is the actual band size different from the predicted?



    This image was generated using the ascites version of the product.

  • Western blot - Anti-Fyn antibody (ab76934)
    Western blot - Anti-Fyn antibody (ab76934)
    All lanes : Anti-Fyn antibody (ab76934) at 5 µg/ml

    Lane 1 : Transfected 293T cell lysate
    Lane 2 : Untransfected 293T cell lysate

    Lysates/proteins at 50 µg per lane.

    Secondary
    All lanes : Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/5000 dilution

    Predicted band size: 60 kDa
    Observed band size: 60 kDa



    This image was generated using the ascites version of the product.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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