Human Heme Oxygenase 1 Matched Antibody Pair Kit (ab215401)
Key features and details
- Unlabeled capture antibody, biotin-labeled detection antibody and calibrated protein standard
- For economical ELISA and ELISA-based assay development
- Reacts with: Human
- Range: 62.5 pg/ml - 4000 pg/ml
Overview
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Product name
Human Heme Oxygenase 1 Matched Antibody Pair Kit
See all Heme Oxygenase 1 kits -
Detection method
Colorimetric -
Assay type
ELISA set -
Sensitivity
2.36 pg/ml -
Range
62.5 pg/ml - 4000 pg/ml -
Species reactivity
Reacts with: Human -
Product overview
Human Heme Oxygenase 1 Matched Antibody Pair Kits include a capture and a biotinylated detector antibody pair, along with a calibrated protein standard, suitable for sandwich ELISA. The Matched Antibody Pair Kit can be used to quantify native and recombinant human Heme Oxygenase 1.
Optimization of the kit reagents to sample type, immunoassay format or instrumentation may be required. Guidelines for use of this kit in a standard 96-well microplate sandwich ELISA using HRP/TMB system of colorimetric detection is described in this assay procedure for the purposes of quantification.
Protocol information and tips on the use of the Matched Antibody Pair kits for sandwich ELISA can be found on our website. An accessory pack can be purchased which includes buffer reagents required to perform 10 x 96-well plate sandwich ELISAs (ab210905).
For additional information on the performance of the antibody pair used in this kit, please see our equivalent SimpleStep ELISA kit ab207621. Please note that while the antibody pair is the same provided in the corresponding SimpleStep ELISA Kit, due to differences in their formulation, this antibody pair cannot be used with the consumables provided with our SimpleStep ELISA Kits.
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Tested applications
Suitable for: IA, ELISAmore details -
Platform
Reagents
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 10 x 96 tests 5 x 96 tests Heme Oxygenase 1 Capture Antibody 2 x 50µg 1 x 50µg Heme Oxygenase 1 Detector Antibody 2 x 12.5µg 1 x 12.5µg Heme Oxygenase 1 Lyophilized Protein 2 vials 1 vial -
Research areas
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Function
Heme oxygenase cleaves the heme ring at the alpha methene bridge to form biliverdin. Biliverdin is subsequently converted to bilirubin by biliverdin reductase. Under physiological conditions, the activity of heme oxygenase is highest in the spleen, where senescent erythrocytes are sequestrated and destroyed. -
Sequence similarities
Belongs to the heme oxygenase family. -
Cellular localization
Microsome. Endoplasmic reticulum. - Information by UniProt
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Alternative names
- 32 kD
- bK286B10
- D8Wsu38e
see all -
Database links
- Entrez Gene: 3162 Human
- Omim: 141250 Human
- SwissProt: P09601 Human
- Unigene: 517581 Human
Images
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Human Heme Oxygenase 1 standard curve.
Standard calibration curve. Background subtracted values are graphed.
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Sandwich ELISA - Human Heme Oxygenase 1 Matched Antibody Pair Kit (ab215401)To learn more about the advantages of recombinant antibodies see here.
