Human Heme Oxygenase 1 ELISA Kit, Fluorescent (ab229429)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 3.5 pg/ml
- Range: 5.86 pg/ml - 6000 pg/ml
- Sample type: Cell culture extracts, Cit plasma, EDTA Plasma, Hep Plasma, Serum, Tissue Extracts
- Detection method: Fluorescent
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human Heme Oxygenase 1 ELISA Kit, Fluorescent
See all Heme Oxygenase 1 kits -
Detection method
Fluorescent -
Precision
Intra-assay Sample n Mean SD CV% Serum 5 3.1% Inter-assay Sample n Mean SD CV% Serum 3 3.8% -
Sample type
Serum, Cell culture extracts, Tissue Extracts, Hep Plasma, EDTA Plasma, Cit plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
3.5 pg/ml -
Range
5.86 pg/ml - 6000 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Serum 87 79% - 94% Cell culture extracts 83 76% - 88% Tissue Extracts 90 86% - 92% EDTA Plasma 85 73% - 95% Cit plasma 85 77% - 93% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Human
Does not react with: Goat, Cow, Pig -
Product overview
Heme Oxygenase 1 (HO 1) in vitro CatchPoint® SimpleStep ELISA® (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Heme Oxygenase 1 (HO 1) protein in human serum, plasma, cell and tissue extracts, and cell culture supernatants.
This CatchPoint SimpleStep ELISA kit has been optimized for Molecular Devices Microplate Readers. Click here for a list of recommended Microplate Readers.
If using a Molecular Devices’ plate reader supported by SoftMax® Pro software, a preconfigured protocol for these CatchPoint SimpleStep ELISA Kits is available with all the protocol and analysis settings at www.softmaxpro.org.The CatchPoint® SimpleStep ELISA® employs an affinity tag labeled capture antibody and a reporter conjugated detector antibody which immunocapture the sample analyte in solution. This entire complex (capture antibody/analyte/detector antibody) is in turn immobilized via immunoaffinity of an anti-tag antibody coating the well. To perform the assay, samples or standards are added to the wells, followed by the antibody mix. After incubation, the wells are washed to remove unbound material. CatchPoint HRP Development Solution containing the Stoplight Red Substrate is added. During incubation, the substrate is catalyzed by HRP generating a fluorescent product. Signal is generated proportionally to the amount of bound analyte and the intensity is measured in a fluorescence plater reader at 530/570/590 nm Excitation/Cutoff/Emission.
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Notes
Heme oxygenase 1 is an enzyme that functions in heme catabolism. The activity of heme oxygenase 1 is to cleave the heme ring to form biliverdin. There are two isozymes of heme oxygenase that have 56% amino acid sequence identity: inducible heme oxygenase 1 and constitutively expressed heme oxygenase 2.
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 100X Stoplight Red Substrate 1 x 120µl 10X Wash Buffer PT (ab206977) 1 x 20ml 500X Hydrogen Peroxide (H2O2, 3%) 1 x 50µl 50X Cell Extraction Enhancer Solution (ab193971) 1 x 1ml 5X Cell Extraction Buffer PTR (ab193970) 1 x 10ml Antibody Diluent 4BR 1 x 6ml Human Heme Oxygenase 1 Capture Antibody (lyophilized) 1 vial Human Heme Oxygenase 1 Detector Antibody (lyophilized) 1 vial Human Heme Oxygenase 1 Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated Black 96-Well Microplate 1 unit Stoplight Red Substrate Buffer 1 x 12ml -
Research areas
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Function
Heme oxygenase cleaves the heme ring at the alpha methene bridge to form biliverdin. Biliverdin is subsequently converted to bilirubin by biliverdin reductase. Under physiological conditions, the activity of heme oxygenase is highest in the spleen, where senescent erythrocytes are sequestrated and destroyed. -
Sequence similarities
Belongs to the heme oxygenase family. -
Cellular localization
Microsome. Endoplasmic reticulum. - Information by UniProt
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Alternative names
- 32 kD
- bK286B10
- D8Wsu38e
see all -
Database links
- Entrez Gene: 3162 Human
- Omim: 141250 Human
- SwissProt: P09601 Human
- Unigene: 517581 Human
Images
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Other - Human Heme Oxygenase 1 ELISA Kit, Fluorescent (ab229429)
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Example of human Heme Oxygenase 1 (HO 1) standard curve in Sample Diluent NS.Background-subtracted data values (mean +/- SD) are graphed.
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Interpolated concentrations of native Heme Oxygenase 1 in human serum and plasma (EDTA) samples.The concentrations of Heme Oxygenase 1 were measured in duplicate, interpolated from the Heme Oxygenase 1 standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 50%, plasma (EDTA) 25%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Heme Oxygenase 1 concentration was determined to be 1.37 ng/mL in serum and 2.12 ng/mL in plasma (EDTA).
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Interpolated concentrations of native Heme Oxygenase.Interpolated concentrations of native Heme Oxygenase 1 in human liver homogenate extract and A549 cell extract based on a 15.6 μg/mL liver homogenate extract and 5 μg/mL A549 cell extract load. The concentrations of Heme Oxygenase 1 were measured in duplicate and interpolated from the Heme Oxygenase 1 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Heme Oxygenase 1 concentration was determined to be 3.30 ng/mL in liver homogenate extract and 1.60 ng/mL in A549 cell extract.
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Serum from ten individual healthy human male donors was measured in duplicate.Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Heme Oxygenase 1 concentration was determined to be 1,630 pg/mL with a range of 391 – 3,198 pg/mL.
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Sandwich ELISA - Human Heme Oxygenase 1 ELISA Kit, Fluorescent (ab229429)To learn more about the advantages of recombinant antibodies see here.
