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Immunology Adaptive Immunity T Cells CD

Anti-CD84 antibody [EPR8325] (ab131256)

Price and availability

304 886 ₸

Availability

Order now and get it on Thursday March 04, 2021

Anti-CD84 antibody [EPR8325] (ab131256)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR8325] to CD84
  • Suitable for: WB, IHC-P
  • Reacts with: Human

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Overview

  • Product name

    Anti-CD84 antibody [EPR8325]
    See all CD84 primary antibodies
  • Description

    Rabbit monoclonal [EPR8325] to CD84
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    WB
    Human
    See all applications and species data
  • Immunogen

    Synthetic peptide within Human CD84 aa 300-400. The exact sequence is proprietary.

  • Positive control

    • Raji and Namalwa cell lysates; Human spleen and tonsil tissues.
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EPR8325
  • Isotype

    IgG
  • Research areas

    • Immunology
    • Adaptive Immunity
    • B Cells
    • CD
    • Stem Cells
    • Hematopoietic Progenitors
    • Surface Molecules

Images

  • Western blot - Anti-CD84 antibody [EPR8325] (ab131256)
    Western blot - Anti-CD84 antibody [EPR8325] (ab131256)
    All lanes : Anti-CD84 antibody [EPR8325] (ab131256) at 1/1000 dilution

    Lane 1 : Raji cell lysate
    Lane 2 : Namalwa cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP labelled Goat anti Rabbit IgG at 1/2000 dilution

    Predicted band size: 39 kDa
    Observed band size: 64-82 kDa
    why is the actual band size different from the predicted?

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD84 antibody [EPR8325] (ab131256)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD84 antibody [EPR8325] (ab131256)

    Immunohistochemical analysis of paraffin embedded Human spleen tissue labelling CD84 with ab131256 antibody at 1/25 dilution.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD84 antibody [EPR8325] (ab131256)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD84 antibody [EPR8325] (ab131256)

    Immunohistochemical analysis of paraffin embedded Human tonsil tissue labelling CD84 with ab131256 antibody at 1/25 dilution.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Anti-CD84 antibody [EPR8325] (ab131256)
    Anti-CD84 antibody [EPR8325] (ab131256)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Alternative products to Anti-CD84 antibody [EPR8325] (ab131256)

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  • Anti-CD84 antibody [153-4D9] - BSA and Azide free (ab212973)

    Applications:

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    Applications:

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    PE Anti-CD84 antibody [CD84.1.21] (ab33218)

    Applications: Flow Cyt

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    Anti-CD84 antibody [EPR8324] (ab137049)

    Applications: Flow Cyt, WB

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