Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-PEX19 antibody [EPR9266(B)] (ab137072)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR9266(B)] to PEX19
  • Suitable for: Flow Cyt, IP, ICC/IF, WB
  • Reacts with: Rat, Human

Overview

  • Product name

    Anti-PEX19 antibody [EPR9266(B)]
    See all PEX19 primary antibodies

  • Description

    Rabbit monoclonal [EPR9266(B)] to PEX19

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    IP
    Human
    WB
    Rat
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide within Human PEX19 aa 1-100. The exact sequence is proprietary.
    Database link: P40855

  • Positive control

    • Jurkat, rat brain lysate, rat heart and MOLT4 cell lysates; Jurkat cells.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

Images

  • Western blot - Anti-PEX19 antibody [EPR9266(B)] (ab137072)
    Western blot - Anti-PEX19 antibody [EPR9266(B)] (ab137072)
    All lanes : Anti-PEX19 antibody [EPR9266(B)] (ab137072) at 1/1000 dilution (Purified)

    Lane 1 : Jurkat (Human T cell leukemia T lymphocyte) whole cell lysates with 5% NFDM/TBST
    Lane 2 : MOLT-4 (Human lymphoblastic leukemia T lymphoblast) whole cell lysates with 5% NFDM/TBST

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

    Predicted band size: 33 kDa
    Observed band size: 35,40 kDa
    why is the actual band size different from the predicted?



    The doublets are reported by PMID: 9339377, 28817674 and 10051604
  • Immunocytochemistry/ Immunofluorescence - Anti-PEX19 antibody [EPR9266(B)] (ab137072)
    Immunocytochemistry/ Immunofluorescence - Anti-PEX19 antibody [EPR9266(B)] (ab137072)

    Immunocytochemistry/ Immunofluorescence analysis of MOLT-4 (Human lymphoblastic leukemia T lymphoblast) cells labeling PEX19 with Purified ab137072 at 1:100 (10.2 µg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1:200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1:1000 (2 µg/ml) dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

  • Flow Cytometry - Anti-PEX19 antibody [EPR9266(B)] (ab137072)
    Flow Cytometry - Anti-PEX19 antibody [EPR9266(B)] (ab137072)

    Flow Cytometry analysis of A549 (Human lung carcinoma epithelial cell) cells labeling PEX19 with purified ab137072 at 1:100 dilution (10 µg/ml) (red). Cells were fixed with 4% Paraformaldehyde. A Goat anti rabbit IgG (Alexa Fluor® 488) secondary antibody was used at 1:2000 dilution. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).

  • Western blot - Anti-PEX19 antibody [EPR9266(B)] (ab137072)
    Western blot - Anti-PEX19 antibody [EPR9266(B)] (ab137072)
    All lanes : Anti-PEX19 antibody [EPR9266(B)] (ab137072) at 1/2000 dilution (Purified)

    Lane 1 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates with 5% NFDM/TBST
    Lane 2 : Rat brain lysates with 5% NFDM/TBST
    Lane 3 : Rat heart lysates with 5% NFDM/TBST

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

    Predicted band size: 33 kDa
    Observed band size: 35,40 kDa why is the actual band size different from the predicted?



    The doublets are reported by PMID: 9339377, 28817674 and 10051604
  • Immunoprecipitation - Anti-PEX19 antibody [EPR9266(B)] (ab137072)
    Immunoprecipitation - Anti-PEX19 antibody [EPR9266(B)] (ab137072)

    ab137072 (purified) at 1:50 dilution (2µg) immunoprecipitating PEX19 in MOLT-4 whole cell lysate.
    Lane 1 (input): MOLT-4 (Human lymphoblastic leukemia T lymphoblast) whole cell lysate 10µg
    Lane 2 (+): ab137072 & MOLT-4 whole cell lysate
    Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab137072 in MOLT-4 whole cell lysate
    For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366) was used for detection at 1:1000 dilution.
    Blocking and diluting buffer: 5% NFDM/TBST.

  • Anti-PEX19 antibody [EPR9266(B)] (ab137072)
    Anti-PEX19 antibody [EPR9266(B)] (ab137072)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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