ab106379, at 2.5 µg/ml, staining Matrilin 4 in Mouse brain tissue by Immunohistochemistry.

ab106379 stained A498 cells. The cells were 100% methanol fixed for 5 minutes at -20°C and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1hour at room temperature to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab106379 at 5µg/ml) overnight at +4°C. The secondary antibody (pseudo-colored green) was Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed (ab150081) used at a 1/1000 dilution for 1hour at room temperature. Alexa Fluor® 594 WGA was used to label plasma membranes (pseudo-colored red) at a 1/200 dilution for 1hour at room temperature. DAPI was used to stain the cell nuclei (pseudo-colored blue) at a concentration of 1.43µM for 1hour at room temperature

Immunofluorescence of Matrilin 4 in mouse brain tissue with ab106379 at 20 ug/mL.

All lanes : Anti-Matrilin 4 antibody (ab106379) at 1 µg/ml

Lane 1 : Rat brain tissue lysate
Lane 2 : Rat brain tissue lysate with blocking peptide

Lysates/proteins at 15 µg per lane.

Predicted band size: Isoform 1, 68 ; Isoform 4, 59 kDa

Immunohistochemical analysis of Human cornea tissue, staining Matrilin 4 wih ab106379.

Tissue was taken from healthy patients (left) or patients with granular dystrophy (right). Sections were blocked with blocking solution for 30 minutes at room temperature before incubation with primary antibody (1/300) overnight at 4°C. Staining was detected with DAB.