Antibodies, Proteins, Kits and Reagents for Life Science

314 937 ₸ Product size

100 µl 314 937 ₸ 10 µl 103 862 ₸

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Anti-p53 antibody [EPR20416-124] (ab246223)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR20416-124] to p53
Suitable for: WB, ICC/IF, Flow Cyt, IP
Reacts with: Mouse

Product name

Anti-p53 antibody [EPR20416-124]
See all p53 primary antibodies
Description

Rabbit monoclonal [EPR20416-124] to p53
Host species

Rabbit

Tested Applications & Species

Application	Species
Flow Cyt	Mouse
ICC/IF	Mouse
IP	Mouse
WB	Mouse

See all applications and species data

Immunogen

Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: LLC1 and RAW 264.7 whole cell lysate. ICC/IF: LLC1 cells. Flow Cyt: LLC1 cells. IP: p53 IP in LLC1 whole cell lysate.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR20416-124
Isotype

IgG
Research areas

Immunocytochemistry/ Immunofluorescence - Anti-p53 antibody [EPR20416-124] (ab246223)

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized LLC1 (mouse lung carcinoma) cells labeling p53 with ab246223 at 1/100 dilution, followed by a AlexaFluor^®488 Goat anti-Rabbit secondary (ab150077, green) at a 1/1000 dilution. Confocal image showing nuclear staining in LLC1 cells. An Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594, ab195889) was used as a counterstain at 1/200 dilution, shown in Red. The nuclear counterstain was DAPI (Blue).

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is AlexaFluor^®488 Goat anti-Rabbit secondary (ab150077) at 1/1000 dilution.
Western blot - Anti-p53 antibody [EPR20416-124] (ab246223)

All lanes : Anti-p53 antibody [EPR20416-124] (ab246223) at 1/1000 dilution

Lane 1 : LLC1 (mouse lung carcinoma) whole cell lysate at 20 µg
Lane 2 : RAW 264.7 (mouse abelson murine leukemia virus-induced tumor macrophage) whole cell lysate at 10 µg

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 43 kDa

Blocking and dilution buffer and concentration: 5% NFDM/TBST.

Exposure time:
Lane 1: 26 seconds.
Lane 2: 81 seconds.

The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 27702985).
Immunoprecipitation - Anti-p53 antibody [EPR20416-124] (ab246223)

p53 was immunoprecipitated from 0.35 mg LLC1 (Mouse lung carcinoma) whole cell lysate with ab246223 at 1/30 dilution. Western blot was performed on the immunoprecipitate using ab246223 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/5000 dilution.

Lane 1: LLC1 whole cell lysate (Input).
Lane 2: ab246223 IP in LLC1 whole cell lysate.
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab246223 in LLC1 whole cell lysate.

Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 3 seconds.
Flow Cytometry - Anti-p53 antibody [EPR20416-124] (ab246223)

Flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol permeabilized, LLC1 (Mouse lung carcinoma) cell line labeling p53 with ab246223 at 1/60 dilution (Red) compared with a Rabbit monoclonal IgG (ab172730, Black) isotype control and an unlabeled control (Cell without incubation with primary antibody and secondary antibody, Blue). A Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077) was used as the secondary antibody at 1/2000 dilution.
Anti-p53 antibody [EPR20416-124] (ab246223)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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