Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-p53 antibody [EPR20416-124] (ab246223)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR20416-124] to p53
  • Suitable for: WB, ICC/IF, Flow Cyt, IP
  • Reacts with: Mouse

Overview

  • Product name

    Anti-p53 antibody [EPR20416-124]
    See all p53 primary antibodies

  • Description

    Rabbit monoclonal [EPR20416-124] to p53

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Mouse
    ICC/IF
    Mouse
    IP
    Mouse
    WB
    Mouse
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: LLC1 and RAW 264.7 whole cell lysate. ICC/IF: LLC1 cells. Flow Cyt: LLC1 cells. IP: p53 IP in LLC1 whole cell lysate.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunocytochemistry/ Immunofluorescence - Anti-p53 antibody [EPR20416-124] (ab246223)
    Immunocytochemistry/ Immunofluorescence - Anti-p53 antibody [EPR20416-124] (ab246223)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized LLC1 (mouse lung carcinoma) cells labeling p53 with ab246223 at 1/100 dilution, followed by a AlexaFluor®488 Goat anti-Rabbit secondary (ab150077, green) at a 1/1000 dilution. Confocal image showing nuclear staining in LLC1 cells. An Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594, ab195889) was used as a counterstain at 1/200 dilution, shown in Red. The nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is AlexaFluor®488 Goat anti-Rabbit secondary (ab150077) at 1/1000 dilution.

     

  • Western blot - Anti-p53 antibody [EPR20416-124] (ab246223)
    Western blot - Anti-p53 antibody [EPR20416-124] (ab246223)
    All lanes : Anti-p53 antibody [EPR20416-124] (ab246223) at 1/1000 dilution

    Lane 1 : LLC1 (mouse lung carcinoma) whole cell lysate at 20 µg
    Lane 2 : RAW 264.7 (mouse abelson murine leukemia virus-induced tumor macrophage) whole cell lysate at 10 µg

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 43 kDa



    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time:
    Lane 1: 26 seconds.
    Lane 2: 81 seconds.

    The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 27702985).

  • Immunoprecipitation - Anti-p53 antibody [EPR20416-124] (ab246223)
    Immunoprecipitation - Anti-p53 antibody [EPR20416-124] (ab246223)

    p53 was immunoprecipitated from 0.35 mg LLC1 (Mouse lung carcinoma) whole cell lysate with ab246223 at 1/30 dilution. Western blot was performed on the immunoprecipitate using ab246223 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/5000 dilution.

    Lane 1: LLC1 whole cell lysate (Input).
    Lane 2: ab246223 IP in LLC1 whole cell lysate.
    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab246223 in LLC1 whole cell lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.
    Exposure time: 3 seconds.

  • Flow Cytometry - Anti-p53 antibody [EPR20416-124] (ab246223)
    Flow Cytometry - Anti-p53 antibody [EPR20416-124] (ab246223)

    Flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol permeabilized, LLC1 (Mouse lung carcinoma) cell line labeling p53 with ab246223 at 1/60 dilution (Red) compared with a Rabbit monoclonal IgG (ab172730, Black) isotype control and an unlabeled control (Cell without incubation with primary antibody and secondary antibody, Blue). A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/2000 dilution.

  • Anti-p53 antibody [EPR20416-124] (ab246223)
    Anti-p53 antibody [EPR20416-124] (ab246223)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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