Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-SET7 antibody [EPR23775-142] (ab274416)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR23775-142] to SET7
  • Suitable for: Flow Cyt, WB
  • Knockout validated
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-SET7 antibody [EPR23775-142]
    See all SET7 primary antibodies

  • Description

    Rabbit monoclonal [EPR23775-142] to SET7

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: NIH/3T3, C6, Hela and Jurkat whole cell lysates. Flow cyt: HeLa cells.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-SET7 antibody [EPR23775-142] (ab274416)
    Western blot - Anti-SET7 antibody [EPR23775-142] (ab274416)
    All lanes : Anti-SET7 antibody [EPR23775-142] (ab274416) at 1/1000 dilution

    Lane 1 : Wild-type HeLa cell lysate
    Lane 2 : SET7 knockout HeLa cell lysate
    Lane 3 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (IRDye® 800CW) (ab216773) and Goat Anti-Mouse IgG H&L (IRDye® 680RD) (ab216776) at 1/10000 dilution

    Predicted band size: 41 kDa
    Observed band size: 50 kDa
    why is the actual band size different from the predicted?



    Blocking and dilution buffer: Intercept® (TBS) Blocking Buffer diluted with an equal volume of 0.1% TBST

    Lanes 1-3: Merged signal (red and green). Green - ab274416 observed at 50 kDa. Red - loading control ab8245 observed at 36 kDa.

    ab274416 Anti-SET7 antibody [EPR23775-142] was shown to specifically react with SET7 in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265770 (knockout cell lysate ab257668) was used. Wild-type and SET7 knockout samples were subjected to SDS-PAGE. ab274416 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated at room temperature for 2. 5 hours at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

  • Flow Cytometry - Anti-SET7 antibody [EPR23775-142] (ab274416)
    Flow Cytometry - Anti-SET7 antibody [EPR23775-142] (ab274416)

    Flow cytometric analysis of 4% paraformaldehyde fixed, 90% methanol permeabilized HepG2 (Human hepatocellular carcinoma epithelial cell, Left) / HeLa (Human cervix adenocarcinoma epithelial cell, RIght) cells labelling SET7 with ab274416 at 1/500 dilution (0.1ug) (Red) compared with a Rabbit monoclonal IgG (ab172730) isotype control (Black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).

    A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.

    Negative control: HepG2 cells.

  • Western blot - Anti-SET7 antibody [EPR23775-142] (ab274416)
    Western blot - Anti-SET7 antibody [EPR23775-142] (ab274416)
    All lanes : Anti-SET7 antibody [EPR23775-142] (ab274416) at 1/1000 dilution

    Lane 1 : HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate
    Lane 2 : HepG2 (human hepatocellular carcinoma epithelial cell) whole cell lysate
    Lane 3 : NIH/3T3 (mouse embryonic fibroblast) whole cell lysate
    Lane 4 : C6 (rat glial tumor glial cell) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/20000 dilution

    Predicted band size: 41 kDa
    Observed band size: 50 kDa why is the actual band size different from the predicted?



    Blocking and dilution buffer: 5% NFDM/TBST.

    Exposure time: 3 minutes.

    Negative control: HepG2 (PMID:27183310).

  • Anti-SET7 antibody [EPR23775-142] (ab274416)
    Anti-SET7 antibody [EPR23775-142] (ab274416)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

© 2021 Astana Biomed Group LLP. All rights reserved.