Anti-SRPK2 antibody [EPR16366] - N-terminal (ab192238)
![Anti-SRPK2 antibody [EPR16366] - N-terminal (ab192238)](https://www.abcam.com/ps/products/192/ab192238/Images/ab192238-230020-anti-srpk2-antibody-epr16366-n-terminal-western-blot.jpg "Anti-SRPK2 antibody [EPR16366] - N-terminal (ab192238)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR16366] to SRPK2 - N-terminal
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Human
Overview
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Product name
Anti-SRPK2 antibody [EPR16366] - N-terminal
See all SRPK2 primary antibodies -
Description
Rabbit monoclonal [EPR16366] to SRPK2 - N-terminal -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF MouseIHC-P HumanWB Human
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- Human fetal brain, fetal kidney, HeLa and HepG2 lysates; Human endometrium adenocarcinoma and kidney tissues; Neuro-2a cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 59% PBS, 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR16366 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-SRPK2 antibody [EPR16366] - N-terminal (ab192238)All lanes : Anti-SRPK2 antibody [EPR16366] - N-terminal (ab192238) at 1/1000 dilution
Lane 1 : HepG2 cell lysate
Lane 2 : HeLa cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution
Predicted band size: 115 kDa
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![Immunocytochemistry/ Immunofluorescence - Anti-SRPK2 antibody [EPR16366] - N-terminal (ab192238)](https://www.abcam.com/ps/products/192/ab192238/Images/ab192238-253281-anti-srpk2-antibody-epr16366-n-terminal-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-SRPK2 antibody [EPR16366] - N-terminal (ab192238)Immunofluorescent analysis of Neuro-2a cells (4% Paraformaldehyde-fixed, 0.1% tritonX-100 permeabilized) labeling SRPK2 with ab192238 at 1/250 dilution (5.4 μg/mL) followed by Goat anti rabbit IgG (AlexaFluor® 488) (ab150077) secondary at 1/200 dilution and counter-stained with DAPI (blue).
Negative controls: anti-SRPK2 at 1/250 dilution, Secondary ab (Goat anti mouse IgG (Alexa Fluor®594)) at 1/500 dilution. -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SRPK2 antibody [EPR16366] - N-terminal (ab192238)](https://www.abcam.com/ps/products/192/ab192238/Images/ab192238-230022-anti-srpk2-antibody-epr16366-n-terminal-immunohistochemistry.gif)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SRPK2 antibody [EPR16366] - N-terminal (ab192238)Immunohistochemical analysis of paraffin-embedded Human endometrium adenocarcinoma tissue labeling SRPK2 with ab192238 at 1/100 dilution followed by pre-diluted HRP Polymer for Rabbit/Mouse IgG secondary antibody and counter-stained with Hematoxylin. (inset: negative control).
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SRPK2 antibody [EPR16366] - N-terminal (ab192238)](https://www.abcam.com/ps/products/192/ab192238/Images/ab192238-230021-anti-srpk2-antibody-epr16366-n-terminal-immunohistochemistry.gif)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SRPK2 antibody [EPR16366] - N-terminal (ab192238)Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling SRPK2 with ab192238 at 1/100 dilution followed by pre-diluted HRP Polymer for Rabbit/Mouse IgG secondary antibody and counter-stained with Hematoxylin. (inset: negative control).
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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![Western blot - Anti-SRPK2 antibody [EPR16366] - N-terminal (ab192238)](https://www.abcam.com/ps/products/192/ab192238/Images/ab192238-230019-anti-srpk2-antibody-epr16366-n-terminal-western-blot.jpg)
Western blot - Anti-SRPK2 antibody [EPR16366] - N-terminal (ab192238)Anti-SRPK2 antibody [EPR16366] - N-terminal (ab192238) at 1/1000 dilution + Human fetal kidney lysate at 10 µg
Secondary
Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution
Predicted band size: 115 kDa
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![Western blot - Anti-SRPK2 antibody [EPR16366] - N-terminal (ab192238)](https://www.abcam.com/ps/products/192/ab192238/Images/ab192238-230018-anti-srpk2-antibody-epr16366-n-terminal-western-blot.jpg)
Western blot - Anti-SRPK2 antibody [EPR16366] - N-terminal (ab192238)Anti-SRPK2 antibody [EPR16366] - N-terminal (ab192238) at 1/2000 dilution + Human fetal brain lysate at 20 µg
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution
Predicted band size: 115 kDa
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![Anti-SRPK2 antibody [EPR16366] - N-terminal (ab192238)](https://www.abcam.com/ps/products/192/ab192238/Images/ab192238-8-benefits-of-recombinant-antibodies.png)
Anti-SRPK2 antibody [EPR16366] - N-terminal (ab192238)
