Anti-MTCO2 antibody [12C4F12] (ab110258)
![Anti-MTCO2 antibody [12C4F12] (ab110258)](https://www.abcam.com/ps/products/110/ab110258/Images/ab110258-123443-anti-mtco2-antibody-12c4f12-western-blot.jpg "Anti-MTCO2 antibody [12C4F12] (ab110258)")
Key features and details
- Mouse monoclonal [12C4F12] to MTCO2
- Suitable for: WB, ICC/IF, Flow Cyt
- Reacts with: Human
- Isotype: IgG2a
Overview
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Product name
Anti-MTCO2 antibody [12C4F12]
See all MTCO2 primary antibodies -
Description
Mouse monoclonal [12C4F12] to MTCO2 -
Host species
Mouse -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt HumanICC/IF HumanWB Human
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Immunogen
Full length native protein (purified). This information is considered to be commercially sensitive.
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Positive control
- WB: Mitochondrial lysate from human heart tissue. ICC/IF: Cultured human embryonic lung derived fibroblasts. Flow Cyt: HeLa cells.
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General notes
This antibody clone is manufactured by Abcam.
Product was previously marketed under the MitoSciences sub-brand.
If you require this antibody in a particular buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com or you can find further information here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
Preservative: 0.02% Sodium azide
Constituent: HEPES buffered saline -
Concentration information loading... -
Purity
IgG fraction -
Purification notes
ab110258 was produced in vitro using hybridomas grown in serum-free medium, and then purified by biochemical fractionation. ab110258 was judged near homogeneity by SDS-PAGE. -
Clonality
Monoclonal -
Clone number
12C4F12 -
Isotype
IgG2a -
Light chain type
kappa -
Research areas
Images
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Western blot - Anti-MTCO2 antibody [12C4F12] (ab110258)Anti-MTCO2 antibody [12C4F12] (ab110258) at 1 µg/ml + Mitochondrial lysate from human heart tissue at 5 µg
Predicted band size: 26 kDa
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![Immunocytochemistry/ Immunofluorescence - Anti-MTCO2 antibody [12C4F12] (ab110258)](https://www.abcam.com/ps/products/110/ab110258/Images/ab110258-123442-anti-mtco2-antibody-12c4f12-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-MTCO2 antibody [12C4F12] (ab110258)Immunocytochemistry/Immunofluorescence analysis of human embryonic lung derived fibroblasts (MRC5) labelling Cytochrome C oxidase subunit II with ab110258 at 5 µg/ml. An Alexa Fluor® 488-conjugated goat anti-mouse IgG2a isotype specific secondary antibody was used at 2 µg/ml.
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![Flow Cytometry - Anti-MTCO2 antibody [12C4F12] (ab110258)](https://www.abcam.com/ps/products/110/ab110258/Images/ab110258-123441-anti-mtco2-antibody-12c4f12-flow-cytometry.jpg)
Flow Cytometry - Anti-MTCO2 antibody [12C4F12] (ab110258)ab110258, at 1 µg/ml, staining Cytochrome C oxidase subunit II in HeLa cells by Flow Cytometry (Blue).An isotype control antibody, at 1 µg/ml, staining in HeLa cells (Red).
