Anti-MTCO2 antibody (ab91317)
Key features and details
- Rabbit polyclonal to MTCO2
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-MTCO2 antibody
See all MTCO2 primary antibodies -
Description
Rabbit polyclonal to MTCO2 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Synthetic peptide corresponding to Human MTCO2 aa 200 to the C-terminus (C terminal) conjugated to keyhole limpet haemocyanin.
(Peptide available asab102090)
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab91317 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
GuaranteedTested applications are guaranteed to work and covered by our Abpromise guarantee.
PredictedPredicted to work for this combination of applications and species but not guaranteed.
IncompatibleDoes not work for this combination of applications and species.
Application Species WB HumanAll applications ChimpanzeeApplication Abreviews Notes WB (1) Use a concentration of 1 µg/ml. Detects a band of approximately 21 kDa (predicted molecular weight: 25 kDa).Notes WB
Use a concentration of 1 µg/ml. Detects a band of approximately 21 kDa (predicted molecular weight: 25 kDa).Target
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Function
Cytochrome c oxidase is the component of the respiratory chain that catalyzes the reduction of oxygen to water. Subunits 1-3 form the functional core of the enzyme complex. Subunit 2 transfers the electrons from cytochrome c via its binuclear copper A center to the bimetallic center of the catalytic subunit 1. -
Involvement in disease
Defects in MT-CO2 are a cause of mitochondrial complex IV deficiency (MT-C4D) [MIM:220110]; also known as cytochrome c oxidase deficiency. A disorder of the mitochondrial respiratory chain with heterogeneous clinical manifestations, ranging from isolated myopathy to severe multisystem disease affecting several tissues and organs. Features include hypertrophic cardiomyopathy, hepatomegaly and liver dysfunction, hypotonia, muscle weakness, excercise intolerance, developmental delay, delayed motor development and mental retardation. A subset of patients manifest Leigh syndrome. -
Sequence similarities
Belongs to the cytochrome c oxidase subunit 2 family. -
Cellular localization
Mitochondrion inner membrane. - Information by UniProt
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Database links
- Entrez Gene: 807864 Chimpanzee
- Entrez Gene: 4513 Human
- Omim: 516040 Human
- SwissProt: P00403 Human
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Alternative names
- COII antibody
- COX 2 antibody
- COX II antibody
see all
Images
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Western blot - Anti-MTCO2 antibody (ab91317)All lanes : Anti-MTCO2 antibody (ab91317) at 1 µg/ml
Lane 1 : Human liver tissue lysate - total protein (ab29889)
Lane 2 : Human heart tissue lysate - total protein (ab29431)
Lane 3 : Human skeletal muscle tissue lysate - total protein (ab29330)
Lane 4 : Human brain tissue lysate - total protein (ab29466)
Lane 5 : Human testis tissue lysate - total protein (ab30257)
Lane 6 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate
Lane 7 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 25 kDa
Observed band size: 21 kDa why is the actual band size different from the predicted?
Additional bands at: 120 kDa, 15 kDa, 65 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 2 minutes
Protocols
Datasheets and documents
References (8)
ab91317 has been referenced in 8 publications.
- Signes A et al. APOPT1/COA8 assists COX assembly and is oppositely regulated by UPS and ROS. EMBO Mol Med 11:N/A (2019). PubMed: 30552096
- Jang SC et al. Mitochondrial protein enriched extracellular vesicles discovered in human melanoma tissues can be detected in patient plasma. J Extracell Vesicles 8:1635420 (2019). PubMed: 31497264
- Reyes A et al. RNase H1 Regulates Mitochondrial Transcription and Translation via the Degradation of 7S RNA. Front Genet 10:1393 (2019). PubMed: 32082360
- Cowan DB et al. Intracoronary Delivery of Mitochondria to the Ischemic Heart for Cardioprotection. PLoS One 11:e0160889 (2016). IHC-P ; Rabbit . PubMed: 27500955
- Zhuang Y et al. Mitochondrial p53 Contributes to Reovirus-Induced Neuronal Apoptosis and Central Nervous System Injury in a Mouse Model of Viral Encephalitis. J Virol 90:7684-91 (2016). IHC-P ; Mouse . PubMed: 27307572
- Spadafora D et al. Methods for Efficient Elimination of Mitochondrial DNA from Cultured Cells. PLoS One 11:e0154684 (2016). WB . PubMed: 27136098
- Kamal RP et al. Emergence of Highly Pathogenic Avian Influenza A(H5N1) Virus PB1-F2 Variants and Their Virulence in BALB/c Mice. J Virol 89:5835-46 (2015). PubMed: 25787281
- Zhao H et al. Myristoylated methionine sulfoxide reductase A protects the heart from ischemia-reperfusion injury. Am J Physiol Heart Circ Physiol 301:H1513-8 (2011). WB ; Mouse . PubMed: 21841012
Images
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Western blot - Anti-MTCO2 antibody (ab91317)All lanes : Anti-MTCO2 antibody (ab91317) at 1 µg/ml
Lane 1 : Human liver tissue lysate - total protein (ab29889)
Lane 2 : Human heart tissue lysate - total protein (ab29431)
Lane 3 : Human skeletal muscle tissue lysate - total protein (ab29330)
Lane 4 : Human brain tissue lysate - total protein (ab29466)
Lane 5 : Human testis tissue lysate - total protein (ab30257)
Lane 6 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate
Lane 7 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 25 kDa
Observed band size: 21 kDa why is the actual band size different from the predicted?
Additional bands at: 120 kDa, 15 kDa, 65 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 2 minutes
