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Anti-MTCO2 antibody [MM7D-5A11CA9] (ab156031)

Key features and details

  • Mouse monoclonal [MM7D-5A11CA9] to MTCO2
  • Suitable for: WB, ICC, Flow Cyt, IP
  • Reacts with: Human, African green monkey
  • Isotype: IgG1

Overview

  • Product name

    Anti-MTCO2 antibody [MM7D-5A11CA9]
    See all MTCO2 primary antibodies

  • Description

    Mouse monoclonal [MM7D-5A11CA9] to MTCO2

  • Host species

    Mouse

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC
    Human
    African green monkey
    IP
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Tissue, cells or virus. This information is considered to be commercially sensitive.

  • Positive control

    • Human liver and heart mitochondrial extracts; Human heart homogenate; 143B(Human osteosarcoma), HeLa and COS7 cells.

  • General notes

    This antibody clone is manufactured by Abcam.

    Product was previously marketed under the MitoSciences sub-brand.

    If you require this antibody in a particular buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com or you can find further information here.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

Images

  • Western blot - Anti-MTCO2 antibody [MM7D-5A11CA9] (ab156031)
    Western blot - Anti-MTCO2 antibody [MM7D-5A11CA9] (ab156031)
    All lanes : Anti-MTCO2 antibody [MM7D-5A11CA9] (ab156031) at 5 µg/ml

    Lane 1 : Human Liver Mitochondria Extract
    Lane 2 : Human Heart Mitochondria Extrac

    Lysates/proteins at 40 µg per lane.

    Secondary
    All lanes : Goat polyclonal to Mouse IgG - AP at 1/3000 dilution

    Predicted band size: 25.5 kDa



    1X Blocking Buffer (ab126587) in H2O was used for blocking and incubation steps.
  • Immunoprecipitation - Anti-MTCO2 antibody [MM7D-5A11CA9] (ab156031)
    Immunoprecipitation - Anti-MTCO2 antibody [MM7D-5A11CA9] (ab156031)
    Immunoprecipitation Coomassie stained gel for Anti-COXII antibody (ab156031).
    Lane 1: Ladder
    Lane 2: COXII Immunoprecipitated from 1mg Human Heart Homogenate (HHH) Extract. ab156031 anti-COXII captures the entire Cytochrome c oxidase complex in immunoprecipitation.
  • Immunocytochemistry - Anti-MTCO2 antibody [MM7D-5A11CA9] (ab156031)
    Immunocytochemistry - Anti-MTCO2 antibody [MM7D-5A11CA9] (ab156031)
    Immunocytochemical analysis of 143B (Human osteosarcoma) wild type (WT) and mtDNA lacking (?0) cells (paraformaldehyde fixed (4%, 20 min) and Triton X-100 permeabilized (0.1%, 15 min)) labeling COXII with ab156031 at 1µg/ml (red) and anti-HSP60 (ab46798) at 1/1000 (green) as a counter stain. Secondary: Alexa 594 goat anti-mouse IgG at 1/1000 and Alexa 488 goat anti-rat IgG at 1/1000. ab156031 shows good reactivity with the wild type (143B-WT) cell line, but shows no signal with the mtDNA lacking (143B-?0) cell line. Cytochrome c oxidase 2 is exclusively encoded by mitochondrial DNA. The anti-HSP60 counter stain antibody identified mitochondria in both cell lines.
  • Immunocytochemistry - Anti-MTCO2 antibody [MM7D-5A11CA9] (ab156031)
    Immunocytochemistry - Anti-MTCO2 antibody [MM7D-5A11CA9] (ab156031)
    Immunocytochemical analysis of COS7 (monkey kidney fibroblast) cells (paraformaldehyde fixed (4%, 20 min) and Triton X-100 permeabilized (0.1%, 15 min)) labeling COXII with ab156031 at 1µg/ml (red). Secondary: Alexa 594 goat anti-mouse IgG at 1/1000. ab156031 shows good reactivity with the COS7 cell type indicating cross reactivity with monkey cytochrome c oxidase.
  • Flow Cytometry - Anti-MTCO2 antibody [MM7D-5A11CA9] (ab156031)
    Flow Cytometry - Anti-MTCO2 antibody [MM7D-5A11CA9] (ab156031)
    Flow cytometric analysis of HeLa cells (fixed and permeabilized with methanol) labeling COXII with ab156031 (blue) at 1µg/ml and an equal amount of an isotype control antibody (red).

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