Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-LYAR antibody [EPR14352-9] (ab182138)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR14352-9] to LYAR
  • Suitable for: WB, ICC/IF, IP
  • Reacts with: Human

Overview

  • Product name

    Anti-LYAR antibody [EPR14352-9]
    See all LYAR primary antibodies

  • Description

    Rabbit monoclonal [EPR14352-9] to LYAR

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    IP
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • Ramos, Jurkat, A431 and K562 cell lysates; K562 cells.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 40% Glycerol (glycerin, glycerine), 59% PBS, 0.05% BSA
  • Concentration information loading...

  • Purity

    Tissue culture supernatant

  • Clonality

    Monoclonal

  • Clone number

    EPR14352-9

  • Isotype

    IgG

  • Research areas

Images

  • Western blot - Anti-LYAR antibody [EPR14352-9] (ab182138)
    Western blot - Anti-LYAR antibody [EPR14352-9] (ab182138)
    All lanes : Anti-LYAR antibody [EPR14352-9] (ab182138) at 1/20000 dilution

    Lane 1 : Ramos cell lysate
    Lane 2 : Jurkat cell lysate
    Lane 3 : A431 cell lysate
    Lane 4 : K562 cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

    Predicted band size: 44 kDa
    Observed band size: 44 kDa

  • Immunoprecipitation - Anti-LYAR antibody [EPR14352-9] (ab182138)
    Immunoprecipitation - Anti-LYAR antibody [EPR14352-9] (ab182138)

    Western blot analysis of immunoprecipitation pellet from K562 cell lysate immunoprecipitated using ab182138 at 1/60 dilution (Lane 1). Lane 2: Negative control.
    Secondary: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 dilution.

  • Immunocytochemistry/ Immunofluorescence - Anti-LYAR antibody [EPR14352-9] (ab182138)
    Immunocytochemistry/ Immunofluorescence - Anti-LYAR antibody [EPR14352-9] (ab182138)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed K562 cells labeling LYAR with ab182138 at 1/100 dilution followed by Goat anti rabbit IgG (Alexa Fluor® 488) secondary at 1/200 dilution. Counter stained with DAPI (blue).

  • Anti-LYAR antibody [EPR14352-9] (ab182138)
    Anti-LYAR antibody [EPR14352-9] (ab182138)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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