Antibodies, Proteins, Kits and Reagents for Life Science

291 484 ₸ Product size

100 µl 291 484 ₸

Order now and get it on Friday March 12, 2021

Anti-LYAR antibody [EPR14352-9] (ab182138)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR14352-9] to LYAR
Suitable for: WB, ICC/IF, IP
Reacts with: Human

Product name

Anti-LYAR antibody [EPR14352-9]
See all LYAR primary antibodies
Description

Rabbit monoclonal [EPR14352-9] to LYAR
Host species

Rabbit

Tested Applications & Species

Application	Species
ICC/IF	Human
IP	Human
WB	Human

See all applications and species data

Immunogen

Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
Positive control
- Ramos, Jurkat, A431 and K562 cell lysates; K562 cells.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 59% PBS, 0.05% BSA
Concentration information loading...
Purity

Tissue culture supernatant
Clonality

Monoclonal
Clone number

EPR14352-9
Isotype

IgG
Research areas

Western blot - Anti-LYAR antibody [EPR14352-9] (ab182138)

All lanes : Anti-LYAR antibody [EPR14352-9] (ab182138) at 1/20000 dilution

Lane 1 : Ramos cell lysate
Lane 2 : Jurkat cell lysate
Lane 3 : A431 cell lysate
Lane 4 : K562 cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

Predicted band size: 44 kDa
Observed band size: 44 kDa
Immunoprecipitation - Anti-LYAR antibody [EPR14352-9] (ab182138)

Western blot analysis of immunoprecipitation pellet from K562 cell lysate immunoprecipitated using ab182138 at 1/60 dilution (Lane 1). Lane 2: Negative control.
Secondary: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 dilution.
Immunocytochemistry/ Immunofluorescence - Anti-LYAR antibody [EPR14352-9] (ab182138)

Immunofluorescent analysis of 4% paraformaldehyde-fixed K562 cells labeling LYAR with ab182138 at 1/100 dilution followed by Goat anti rabbit IgG (Alexa Fluor® 488) secondary at 1/200 dilution. Counter stained with DAPI (blue).
Anti-LYAR antibody [EPR14352-9] (ab182138)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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