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Anti-CD31 antibody [EPR17260-254] - Low endotoxin, Azide free (ab224260)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR17260-254] to CD31 - Low endotoxin, Azide free
  • Suitable for: WB, Sandwich ELISA, IP
  • Reacts with: Mouse

Overview

  • Product name

    Anti-CD31 antibody [EPR17260-254] - Low endotoxin, Azide free
    See all CD31 primary antibodies

  • Description

    Rabbit monoclonal [EPR17260-254] to CD31 - Low endotoxin, Azide free

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IP
    Mouse
    sELISA
    Mouse
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Mouse CD31 active protein; mouse and rat platelet and lung lysates; bEnd.3 whole cell lysate. IP: bEnd.3 whole cell lysate.

  • General notes

    ab224260 is a carrier-free antibody designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our Low endotoxin, azide-free formats have low endotoxin level (≤ 1 EU/ml, determined by the LAL assay) and are free from azide, to achieve consistent experimental results in functional assays.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Images

  • Immunoprecipitation - Anti-CD31 antibody [EPR17260-254] - Low endotoxin, Azide free (ab224260)
    Immunoprecipitation - Anti-CD31 antibody [EPR17260-254] - Low endotoxin, Azide free (ab224260)

    CD31 was immunoprecipitated from 1 mg of bEnd.3 (Mouse brain endothelioma cell line) whole cell lysate with ab213175 at 1/40 dilution.

    Western blot was performed from the immunoprecipitate using ab213175 at 1/1000 dilution.

    VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.

    Lane 1: bEnd.3 whole cell lysate 10µg (Input). Lane 2: ab213175 IP in bEnd.3 whole cell lysate. Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab213175 in bEnd.3 whole cell lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 10 seconds.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab213175).

  • Sandwich ELISA - Anti-CD31 antibody [EPR17260-254] - Low endotoxin, Azide free (ab224260)
    Sandwich ELISA - Anti-CD31 antibody [EPR17260-254] - Low endotoxin, Azide free (ab224260)

    This ELISA data was generated using the same anti-CD31 antibody clone [EPR17260-254] in a different buffer formulation (cat# ab213175).

    Example of mouse CD31 standard curve in 1X Cell Extraction Buffer PTR using recombinant CD31 antibody (ab213175) and a recombinant monoclonal antibody. Background-subtracted data values (mean +/- SD) are graphed.

    For a matched antibody pair set, see Mouse CD31 Matched Antibody Pair Kit (ab212065).

    For a complete ELISA kit, see Mouse CD31 ELISA kit (ab204527).

  • Anti-CD31 antibody [EPR17260-254] - Low endotoxin, Azide free (ab224260)
    Anti-CD31 antibody [EPR17260-254] - Low endotoxin, Azide free (ab224260)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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