Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Quiescin Q6 antibody [EPR21866] - BSA and Azide free (ab237600)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR21866] to Quiescin Q6 - BSA and Azide free
  • Suitable for: IHC-P, WB, IP, Flow Cyt
  • Reacts with: Human

Overview

  • Product name

    Anti-Quiescin Q6 antibody [EPR21866] - BSA and Azide free
    See all Quiescin Q6 primary antibodies

  • Description

    Rabbit monoclonal [EPR21866] to Quiescin Q6 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IHC-P, WB, IP, Flow Cytmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • IHC-P: Human pancreatic cancer tissue.

  • General notes

    Ab237600 is the carrier-free version of ab235444. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab237600 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.

  • Storage buffer

    pH: 7.2
    Constituent: PBS

  • Carrier free

    Yes
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR21866

  • Isotype

    IgG

  • Research areas

Images

  • Immunoprecipitation - Anti-Quiescin Q6 antibody [EPR21866] - BSA and Azide free (ab237600)
    Immunoprecipitation - Anti-Quiescin Q6 antibody [EPR21866] - BSA and Azide free (ab237600)

    Quiescin Q6 was immunoprecipitated from 0.35 mg of MDA-MB-231 (Human breast adenocarcinoma cell line) whole cell lysate with ab235444 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab235444 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/5000 dilution.

    Lane 1: MDA-MB-231 whole cell lysate 10 μg (Input).
    Lane 2: ab235444 IP in MDA-MD-231 whole cell lysate.
    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab235444 in MDA-MD-231 whole cell lysate.

    Blocking/Dilution buffer: 5% NFDM/TBST.
    Exposure time: 30 seconds.

    Quiescin Q6 has two isoforms due to expression from a long (Quiescin Q6-L) and a short (Quiescin Q6-S) transcript.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab235444).

  • Flow Cytometry - Anti-Quiescin Q6 antibody [EPR21866] - BSA and Azide free (ab237600)
    Flow Cytometry - Anti-Quiescin Q6 antibody [EPR21866] - BSA and Azide free (ab237600)

    Flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol-permeabilized MDA-MB-231 (Human breast adenocarcinoma cell line) cells labeling Quiescin Q6 with ab235444 at 1/60 (red) compared with a Rabbit monoclonal IgG (ab172730) (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077), at 1/2000 dilution was used as the secondary antibody.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab235444).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Quiescin Q6 antibody [EPR21866] - BSA and Azide free (ab237600)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Quiescin Q6 antibody [EPR21866] - BSA and Azide free (ab237600)

    Immunohistochemical analysis of paraffin-embedded human breast ductal cancer tissue labeling Quiescin Q6 with ab235444 at 1/200 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Cytoplasmic staining in human breast ductal cancer (PMID: 23536962) is observed. Counter stained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.

    Heat-mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab235444).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Quiescin Q6 antibody [EPR21866] - BSA and Azide free (ab237600)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Quiescin Q6 antibody [EPR21866] - BSA and Azide free (ab237600)

    Immunohistochemical analysis of paraffin-embedded human pancreatic cancer tissue labeling Quiescin Q6 with ab235444 at 1/200 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Cytoplasmic staining in human pancreatic cancer (PMID: 19795908, 21989104) is observed. Counter stained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.

    Heat-mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab235444).

  • Anti-Quiescin Q6 antibody [EPR21866] - BSA and Azide free (ab237600)
    Anti-Quiescin Q6 antibody [EPR21866] - BSA and Azide free (ab237600)

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