Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-LMO2 antibody [EPR22404-45] - BSA and Azide free (ab254270)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR22404-45] to LMO2 - BSA and Azide free
  • Suitable for: IHC-P
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-LMO2 antibody [EPR22404-45] - BSA and Azide free
    See all LMO2 primary antibodies

  • Description

    Rabbit monoclonal [EPR22404-45] to LMO2 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IHC-Pmore details
    Unsuitable for: Flow Cyt,ICC/IF,IP or WB

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • IHC-P: Human tonsil and diffuse large B-cell lymphoma tissue; Mouse and rat colon tissue.

  • General notes

    Ab254270 is the carrier-free version of ab245250. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab254270 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.

  • Storage buffer

    pH: 7.2
    Constituent: PBS

  • Carrier free

    Yes
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR22404-45

  • Isotype

    IgG

  • Research areas

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LMO2 antibody [EPR22404-45] - BSA and Azide free (ab254270)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LMO2 antibody [EPR22404-45] - BSA and Azide free (ab254270)

    Immunohistochemical analysis of paraffin-embedded rat colon tissue labeling LM02 with ab245250 at 1/1000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Nuclear staining in endothelial cells of rat colon (PMID: 19141387) is onserved. Counterstained with hematoxylin. Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab245250).

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LMO2 antibody [EPR22404-45] - BSA and Azide free (ab254270)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LMO2 antibody [EPR22404-45] - BSA and Azide free (ab254270)

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue labeling LM02 with ab245250 at 1/1000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Nuclear staining in endothelial cells of mouse colon (PMID: 19141387) is observed. Counterstained with hematoxylin. Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab245250).

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LMO2 antibody [EPR22404-45] - BSA and Azide free (ab254270)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LMO2 antibody [EPR22404-45] - BSA and Azide free (ab254270)

    Immunohistochemical analysis of paraffin-embedded human diffuse large B-cell lymphoma tissue labeling LM02 with ab245250 at 1/1000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Nuclear staining in human diffuse large B-cell lymphoma (PMID: 17038524) is observed. Counterstained with hematoxylin. Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab245250).

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LMO2 antibody [EPR22404-45] - BSA and Azide free (ab254270)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LMO2 antibody [EPR22404-45] - BSA and Azide free (ab254270)

    Immunohistochemical analysis of paraffin-embedded human tonsil tissue labeling LM02 with ab245250 at 1/1000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Nuclear staining in germinal center of human tonsil (PMID: 17038524) is observed. Counterstained with hematoxylin. Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab245250).

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Anti-LMO2 antibody [EPR22404-45] - BSA and Azide free (ab254270)
    Anti-LMO2 antibody [EPR22404-45] - BSA and Azide free (ab254270)

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