Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-delta 1 Catenin/CAS antibody [EPR357(2)] - BSA and Azide free (ab239916)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR357(2)] to delta 1 Catenin/CAS - BSA and Azide free
  • Suitable for: Flow Cyt (Intra), IHC-P, ICC/IF, WB
  • Reacts with: Mouse, Human

Overview

  • Product name

    Anti-delta 1 Catenin/CAS antibody [EPR357(2)] - BSA and Azide free
    See all delta 1 Catenin/CAS primary antibodies

  • Description

    Rabbit monoclonal [EPR357(2)] to delta 1 Catenin/CAS - BSA and Azide free

  • Host species

    Rabbit

  • Specificity

    The mouse recommendation is based on the WB results. We do not guarantee IHC-P for mouse.

  • Tested applications

    Suitable for: Flow Cyt (Intra), IHC-P, ICC/IF, WBmore details

  • Species reactivity

    Reacts with: Mouse, Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    ab239916 is the carrier-free version of ab92514.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

Images

  • Immunocytochemistry/ Immunofluorescence - Anti-delta 1 Catenin/CAS antibody [EPR357(2)] - BSA and Azide free (ab239916)
    Immunocytochemistry/ Immunofluorescence - Anti-delta 1 Catenin/CAS antibody [EPR357(2)] - BSA and Azide free (ab239916)

    Immunocytochemistry/ Immunofluorescence analysis of A431 (Human epidermoid carcinoma epithelial cell) cells labeling delta 1 Catenin/CAS with purified ab92514 at 1:50 dilution (2.2 µg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1:200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1:1000 (2 µg/ml) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab92514).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-delta 1 Catenin/CAS antibody [EPR357(2)] - BSA and Azide free (ab239916)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-delta 1 Catenin/CAS antibody [EPR357(2)] - BSA and Azide free (ab239916)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human ovarian cancer tissue sections labeling delta 1 Catenin/CAS with purified ab92514 at 1:50 dilution (1.84 µg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab92514).

  • Flow Cytometry - Anti-delta 1 Catenin/CAS antibody [EPR357(2)] - BSA and Azide free (ab239916)
    Flow Cytometry - Anti-delta 1 Catenin/CAS antibody [EPR357(2)] - BSA and Azide free (ab239916)

    Flow Cytometry analysis of A431 (human epidermoid carcinoma) cells labeling delta 1 Catenin/CAS with unpurified ab92514 at 1/20 dilution (10 µg/ml) (Red). Cells were fixed with 4% paraformaldehyde and permeabilized with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor® 488) (1/2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Black) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) was used as the unlabeled control.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab92514).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-delta 1 Catenin/CAS antibody [EPR357(2)] - BSA and Azide free (ab239916)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-delta 1 Catenin/CAS antibody [EPR357(2)] - BSA and Azide free (ab239916)

    Paraffin embedded human breast carcinoma tissue (panel 1) and human colonic adenocarcinoma tissue (panel 2) labeled with unpurified ab92514 at 1/100 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab92514).

    Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunocytochemistry/ Immunofluorescence - Anti-delta 1 Catenin/CAS antibody [EPR357(2)] - BSA and Azide free (ab239916)
    Immunocytochemistry/ Immunofluorescence - Anti-delta 1 Catenin/CAS antibody [EPR357(2)] - BSA and Azide free (ab239916)

    A431 (Human epidermoid carcinoma cell line) cells stained for delta 1 Catenin/CAS using unpurified ab92514 at a dilution of 1/100 in ICC/IF.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab92514).

  • OI-RD Scanning - Anti-delta 1 Catenin/CAS antibody [EPR357(2)] - BSA and Azide free (ab239916)
    OI-RD Scanning - Anti-delta 1 Catenin/CAS antibody [EPR357(2)] - BSA and Azide free (ab239916)
    Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab92514).

  • Anti-delta 1 Catenin/CAS antibody [EPR357(2)] - BSA and Azide free (ab239916)
    Anti-delta 1 Catenin/CAS antibody [EPR357(2)] - BSA and Azide free (ab239916)

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