Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-LYRIC/AEG1 antibody [EPR20797] - BSA and Azide free (ab229128)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR20797] to LYRIC/AEG1 - BSA and Azide free
  • Suitable for: WB, ICC/IF, IP, Flow Cyt, IHC-P
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-LYRIC/AEG1 antibody [EPR20797] - BSA and Azide free
    See all LYRIC/AEG1 primary antibodies

  • Description

    Rabbit monoclonal [EPR20797] to LYRIC/AEG1 - BSA and Azide free

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    IHC-P
    Human
    IP
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • IHC-P: Human astrocytoma tissue.

  • General notes

    ab229128 is the carrier-free version of ab227981 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    Ab229128 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product was previously labelled as LYRIC

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LYRIC/AEG1 antibody [EPR20797] - BSA and Azide free (ab229128)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LYRIC/AEG1 antibody [EPR20797] - BSA and Azide free (ab229128)

    Immunohistochemical analysis of paraffin-embedded human ovarian cancer tissue labeling LYRIC/AEG1 with ab227981 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Cytoplasmic staining in human ovarian cancer tissue (PMID: 27143933) is observed. Counter stained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab227981).

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LYRIC/AEG1 antibody [EPR20797] - BSA and Azide free (ab229128)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LYRIC/AEG1 antibody [EPR20797] - BSA and Azide free (ab229128)

    Immunohistochemical analysis of paraffin-embedded mouse cerebrum tissue labeling LYRIC/AEG1 with ab227981 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Cytoplasmic staining in mouse cerebrum tissue (PMID:25197376) is observed. Counter stained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab227981).

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LYRIC/AEG1 antibody [EPR20797] - BSA and Azide free (ab229128)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LYRIC/AEG1 antibody [EPR20797] - BSA and Azide free (ab229128)

    Immunohistochemical analysis of paraffin-embedded rat cerebrum tissue labeling LYRIC/AEG1 with ab227981 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Cytoplasmic staining in rat cerebrum tissue (PMID:25197376) is observed. Counter stained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab227981).

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunocytochemistry/ Immunofluorescence - Anti-LYRIC/AEG1 antibody [EPR20797] - BSA and Azide free (ab229128)
    Immunocytochemistry/ Immunofluorescence - Anti-LYRIC/AEG1 antibody [EPR20797] - BSA and Azide free (ab229128)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (human epithelial cell line from cervix adenocarcinoma) cells labeling LYRIC/AEG1 with ab227981 at 1/100 dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic and weakly nuclear staining in HeLa cell line (PMID: 21750868).

    The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) (red) at 1/200 dilution.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab227981).

  • Immunocytochemistry/ Immunofluorescence - Anti-LYRIC/AEG1 antibody [EPR20797] - BSA and Azide free (ab229128)
    Immunocytochemistry/ Immunofluorescence - Anti-LYRIC/AEG1 antibody [EPR20797] - BSA and Azide free (ab229128)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized PC-3 (human prostate adenocarcinoma cell line) cells labeling LYRIC/AEG1 with ab227981 at 1/100 dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic staining in PC-3 cell line.

    The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) (red) at 1/200 dilution.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab227981).

  • Flow Cytometry - Anti-LYRIC/AEG1 antibody [EPR20797] - BSA and Azide free (ab229128)
    Flow Cytometry - Anti-LYRIC/AEG1 antibody [EPR20797] - BSA and Azide free (ab229128)

    Flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol-permeabilized HeLa (human epithelial cell line from cervix adenocarcinoma) cell line labeling LYRIC/AEG1 with ab227981 at 1/500 dilution (red) compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) at 1/2000 dilution was used as the secondary antibody.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab227981).

  • Immunoprecipitation - Anti-LYRIC/AEG1 antibody [EPR20797] - BSA and Azide free (ab229128)
    Immunoprecipitation - Anti-LYRIC/AEG1 antibody [EPR20797] - BSA and Azide free (ab229128)

    LYRIC/AEG1 was immunoprecipitated from 0.35 mg of HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate with ab227981 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab227981 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.

    Lane 1: HeLa whole cell lysate 10 µg (Input). 

    Lane 2: ab227981 IP in HeLa whole cell lysate. 

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab227981 in HeLa whole cell lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 1 second.

    The protein migrates as a 75/80kDa doublet, as has been observed in the literature (PMID: 23835593).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab227981).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LYRIC/AEG1 antibody [EPR20797] - BSA and Azide free (ab229128)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LYRIC/AEG1 antibody [EPR20797] - BSA and Azide free (ab229128)

    Immunohistochemical analysis of paraffin-embedded human astrocytoma tissue labeling LYRIC/AEG1 with ab227981 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Cytoplasmic staining in human astrocytoma tissue (PMID: 25197376) is observed. Counter stained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab227981).

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Anti-LYRIC/AEG1 antibody [EPR20797] - BSA and Azide free (ab229128)
    Anti-LYRIC/AEG1 antibody [EPR20797] - BSA and Azide free (ab229128)

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