Antibodies, Proteins, Kits and Reagents for Life Science

278 083 ₸ Product size

100 µl 278 083 ₸ 10 µl 110 563 ₸

Order now and get it on Tuesday March 09, 2021

Anti-LYRIC/AEG1 antibody [EPR20797] (ab227981)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR20797] to LYRIC/AEG1
Suitable for: WB, ICC/IF, IP, Flow Cyt, IHC-P
Reacts with: Mouse, Rat, Human

Product name

Anti-LYRIC/AEG1 antibody [EPR20797]
See all LYRIC/AEG1 primary antibodies
Description

Rabbit monoclonal [EPR20797] to LYRIC/AEG1
Host species

Rabbit

Tested Applications & Species

Application	Species
Flow Cyt	Human
ICC/IF	Human
IHC-P	Mouse Rat Human
IP	Human
WB	Rat

See all applications and species data

Immunogen

Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: MCF7, T47D, HeLa, PC-3 and PC-12 whole cell lysates; Human lung cancer lysate. IHC-P: Human astrocytoma and ovarian cancer tissues; Mouse and rat cerebrum tissues. ICC/IF: HeLa and PC-3 cells. Flow Cyt: HeLa cells. IP: HeLa whole cell lysate.
General notes
This product was previously labelled as LYRIC

This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 0.05% BSA, 40% Glycerol (glycerin, glycerine), PBS
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR20797
Isotype

IgG
Research areas

Western blot - Anti-LYRIC/AEG1 antibody [EPR20797] (ab227981)

All lanes : Anti-LYRIC/AEG1 antibody [EPR20797] (ab227981) at 1/1000 dilution

Lane 1 : MCF7 (human breast adenocarcinoma cell line) whole cell lysate at 20 µg
Lane 2 : T-47D (human ductal breast epithelial tumor cell line) whole cell lysate at 20 µg
Lane 3 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 20 µg
Lane 4 : PC-3 (human prostate adenocarcinoma cell line) whole cell lysate at 20 µg
Lane 5 : Human lung cancer lysate at 10 µg
Lane 6 : PC-12 (rat adrenal gland pheochromocytoma cell line) whole cell lysate at 10 µg

Secondary
Lanes 1-4 & 6 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Lane 5 : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/4000 dilution

Predicted band size: 64 kDa
Observed band size: 75,80 kDa
why is the actual band size different from the predicted?

Blocking/Dilution buffer: 5% NFDM/TBST.

Exposure times.

Lanes 1, 2 & 5: 3 minutes.

Lanes 3 & 4: 15 seconds.

Lane 6: 58 seconds.

The blot was developed on a BIO-RAD® ChemiDoc™ MP instrument.

This antibody detected LYRIC/AEG1 in rat PC-12 cells only, not in other rat tissues or cell lines tested.

The protein migrates as a 75/80kDa doublet, as has been observed in the literature (PMID: 23835593).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LYRIC/AEG1 antibody [EPR20797] (ab227981)

Immunohistochemical analysis of paraffin-embedded human ovarian cancer tissue labeling LYRIC/AEG1 with ab227981 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Cytoplasmic staining in human ovarian cancer tissue (PMID: 27143933) is observed. Counter stained with hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunocytochemistry/ Immunofluorescence - Anti-LYRIC/AEG1 antibody [EPR20797] (ab227981)

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (human epithelial cell line from cervix adenocarcinoma) cells labeling LYRIC/AEG1 with ab227981 at 1/100 dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic and weakly nuclear staining in HeLa cell line (PMID: 21750868).

The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594) (ab195889) (red) at 1/200 dilution.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LYRIC/AEG1 antibody [EPR20797] (ab227981)

Immunohistochemical analysis of paraffin-embedded human astrocytoma tissue labeling LYRIC/AEG1 with ab227981 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Cytoplasmic staining in human astrocytoma tissue (PMID: 25197376) is observed. Counter stained with hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LYRIC/AEG1 antibody [EPR20797] (ab227981)

Immunohistochemical analysis of paraffin-embedded mouse cerebrum tissue labeling LYRIC/AEG1 with ab227981 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Cytoplasmic staining in mouse cerebrum tissue (PMID:25197376) is observed. Counter stained with hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LYRIC/AEG1 antibody [EPR20797] (ab227981)

Immunohistochemical analysis of paraffin-embedded rat cerebrum tissue labeling LYRIC/AEG1 with ab227981 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Cytoplasmic staining in rat cerebrum tissue (PMID:25197376) is observed. Counter stained with hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunocytochemistry/ Immunofluorescence - Anti-LYRIC/AEG1 antibody [EPR20797] (ab227981)

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized PC-3 (human prostate adenocarcinoma cell line) cells labeling LYRIC/AEG1 with ab227981 at 1/100 dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic staining in PC-3 cell line.

The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594) (ab195889) (red) at 1/200 dilution.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution.
Flow Cytometry - Anti-LYRIC/AEG1 antibody [EPR20797] (ab227981)

Flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol-permeabilized HeLa (human epithelial cell line from cervix adenocarcinoma) cell line labeling LYRIC/AEG1 with ab227981 at 1/500 dilution (red) compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) at 1/2000 dilution was used as the secondary antibody.
Immunoprecipitation - Anti-LYRIC/AEG1 antibody [EPR20797] (ab227981)

LYRIC/AEG1 was immunoprecipitated from 0.35 mg of HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate with ab227981 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab227981 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.

Lane 1: HeLa whole cell lysate 10 µg (Input).

Lane 2: ab227981 IP in HeLa whole cell lysate.

Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab227981 in HeLa whole cell lysate.

Blocking and dilution buffer and concentration: 5% NFDM/TBST.

Exposure time: 1 second.

The protein migrates as a 75/80kDa doublet, as has been observed in the literature (PMID: 23835593).
Anti-LYRIC/AEG1 antibody [EPR20797] (ab227981)

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