Anti-delta 1 Catenin/CAS antibody [SP63] - C-terminal (ab227638)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [SP63] to delta 1 Catenin/CAS - C-terminal
- Suitable for: IHC-P, Flow Cyt, ICC/IF
- Reacts with: Human
Overview
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Product name
Anti-delta 1 Catenin/CAS antibody [SP63] - C-terminal
See all delta 1 Catenin/CAS primary antibodies -
Description
Rabbit monoclonal [SP63] to delta 1 Catenin/CAS - C-terminal -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt HumanICC/IF HumanIHC-P Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- IHC-P: Human breast carcinoma, breast ductal cell carcinoma, bladder transitional cell carcinoma, cervical squamous cell carcinoma, cervix, colon adenocarcinoma, endometrium adenocarcinoma, hepatocellular carcinoma, stomach adenocarcinoma, prostate adenocarcinoma and esophagus tissues. ICC/IF: A431 cells. Flow Cyt: A431 cells.
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General notes
This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.60
Preservative: 0.1% Sodium azide
Constituents: PBS, 1% BSA -
Concentration information loading...
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Purity
Protein A/G purified -
Purification notes
Purified from TCS by protein A/G. -
Clonality
Monoclonal -
Clone number
SP63 -
Isotype
IgG -
Research areas
Images
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Immunocytochemistry/ Immunofluorescence analysis of A431 (human epidermoid carcinoma epithelial cell) cells labeling delta 1 Catenin/CAS with purified ab227638. Cells were fixed in 100% Methanol. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody. DAPI was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
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Formalin-fixed, paraffin-embedded human breast carcinoma tissue stained for delta 1 Catenin/CAS using ab227638 at 1/100 dilution in immunohistochemical analysis.
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Flow cytometry analysis of A431 (human epidermoid carcinoma epithelial cell) labeling delta 1 Catenin/CAS with purified ab227638 at (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as a secondary antibody. Isotype control -Rabbit monoclonal IgG (ab172730) / Black. Unlableled control -Unlabelled cells / blue.
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Formalin-fixed, paraffin-embedded human breast ductal cell carcinoma tissue stained for delta 1 Catenin/CAS using ab227638 at 1/100 dilution in immunohistochemical analysis.
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Formalin-fixed, paraffin-embedded human bladder transitional cell carcinoma tissue stained for delta 1 Catenin/CAS using ab227638 at 1/100 dilution in immunohistochemical analysis.
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Formalin-fixed, paraffin-embedded human cervical squamous cell carcinoma tissue stained for delta 1 Catenin/CAS using ab227638 at 1/100 dilution in immunohistochemical analysis.
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Formalin-fixed, paraffin-embedded human cervix tissue stained for delta 1 Catenin/CAS using ab227638 at 1/100 dilution in immunohistochemical analysis.
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Formalin-fixed, paraffin-embedded human colon adenocarcinoma tissue stained for delta 1 Catenin/CAS using ab227638 at 1/100 dilution in immunohistochemical analysis.
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Formalin-fixed, paraffin-embedded human endometrium adenocarcinoma tissue stained for delta 1 Catenin/CAS using ab227638 at 1/100 dilution in immunohistochemical analysis.
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Formalin-fixed, paraffin-embedded human hepatocellular carcinoma tissue stained for delta 1 Catenin/CAS using ab227638 at 1/100 dilution in immunohistochemical analysis.
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Formalin-fixed, paraffin-embedded human stomach adenocarcinoma tissue stained for delta 1 Catenin/CAS using ab227638 at 1/100 dilution in immunohistochemical analysis.
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Formalin-fixed, paraffin-embedded human esophagus tissue stained for delta 1 Catenin/CAS using ab227638 at 1/100 dilution in immunohistochemical analysis.
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Formalin-fixed, paraffin-embedded human prostate adenocarcinoma tissue stained for delta 1 Catenin/CAS using ab227638 at 1/100 dilution in immunohistochemical analysis.
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