Antibodies, Proteins, Kits and Reagents for Life Science

Anti-delta 1 Catenin/CAS antibody [YE372] (ab32095)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [YE372] to delta 1 Catenin/CAS
Suitable for: WB, IHC-P, Flow Cyt, IP
Reacts with: Human

Product name

Anti-delta 1 Catenin/CAS antibody [YE372]
See all delta 1 Catenin/CAS primary antibodies
Description

Rabbit monoclonal [YE372] to delta 1 Catenin/CAS
Host species

Rabbit
Specificity

This antibody is specific for human delta 1 Catenin/CAS (isoform 1ABC and 968AA). It also detects splice isoforms 1, 1A, 1B, 1C, 1AB, 1AC, 1BC, 2, 2A, 2B, 2C, 2AB, 2AC, 2BC and 2ABC of delta 1 Catenin/CAS.

Tested Applications & Species

Application	Species
Flow Cyt	Human
IHC-P	Human
IP	Human
WB	Human

See all applications and species data

Immunogen

Synthetic peptide within Human delta 1 Catenin/CAS aa 50-150 (N terminal). The exact sequence is proprietary.
Epitope

ab32095 reacts with an epitope located in the N terminal region of delta 1 Catenin
Positive control
- HeLa whole cell lysate (ab150035). human breast carcinoma
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer

pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 49% PBS, 50% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

YE372
Isotype

IgG
Research areas

Western blot - Anti-delta 1 Catenin/CAS antibody [YE372] (ab32095)

Anti-delta 1 Catenin/CAS antibody [YE372] (ab32095) at 1/5000 dilution + Hela cell lysate.

Predicted band size: 108 kDa
Observed band size: 120 kDa
why is the actual band size different from the predicted?
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-delta 1 Catenin/CAS antibody [YE372] (ab32095)

ab32095 at a 1/100 dilution staining delta 1 Catenin/CAS in human breast carcinoma, using Immunohistochemistry, Paraffin Embedded Tissue.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Flow Cytometry - Anti-delta 1 Catenin/CAS antibody [YE372] (ab32095)

Flow Cytometry analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling delta 1 Catenin/CAS with purified ab32095 at 1/100 dilution (10 µg/mL) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cells without incubation with primary antibody and secondary antibody (Blue).
Immunoprecipitation - Anti-delta 1 Catenin/CAS antibody [YE372] (ab32095)

Purified ab32095 at 1/50 dilution (2µg) immunoprecipitating delta 1 Catenin/CAS in HeLa whole cell lysate.
Lane 1 (input): HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate 10µg
Lane 2 (+): ab32095 + HeLa whole cell lysate.
Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab32095 in HeLa whole cell lysate.
VeriBlot for IP Detection Reagent (HRP) (ab131366) (1/1000 dilution) was used for Western blotting.
Blocking Buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM/TBST.
Observed band size: 120 kDa
Multi-bands could be isoforms of delta 1 Catenin based on Uniprot.
Anti-delta 1 Catenin/CAS antibody [YE372] (ab32095)

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