Anti-delta 1 Catenin/CAS (phospho Y228) antibody [E205] (ab32403)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [E205] to delta 1 Catenin/CAS (phospho Y228)
- Suitable for: WB, IHC-P
- Reacts with: Human
Overview
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Product name
Anti-delta 1 Catenin/CAS (phospho Y228) antibody [E205]
See all delta 1 Catenin/CAS primary antibodies -
Description
Rabbit monoclonal [E205] to delta 1 Catenin/CAS (phospho Y228) -
Host species
Rabbit -
Specificity
This antibody only detects delta 1 Catenin/CAS phosphorylated on Tyrosine 228.
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Tested Applications & Species
See all applications and species dataApplication Species WB Human -
Immunogen
Synthetic peptide corresponding to Human delta 1 Catenin/CAS (phospho Y228).
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Positive control
- WB: HeLa whole cell lysate (ab150035). IHC-P: Breast carcinoma.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 49% PBS, 50% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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Clonality
Monoclonal -
Clone number
E205 -
Isotype
IgG -
Research areas
Images
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All lanes : Anti-delta 1 Catenin/CAS (phospho Y228) antibody [E205] (ab32403)
Lane 1 : HeLa cell lysate, untreated.
Lane 2 : HeLa cell lysate, treated with Pervanandate.
Lane 3 : HeLa cell lysate, treated with Phosphatase.
Predicted band size: 108 kDa
Observed band size: 115 kDa why is the actual band size different from the predicted?
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Ab32403, at a 1/50 dilution, staining delta 1 Catenin/CAS in paraffin embedded breast carcinoma tissue sections by Immunohistochemistry.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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