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Signal Transduction Cytoskeleton / ECM Cytoskeleton Microfilaments Actin etc Catenins

Anti-delta 1 Catenin/CAS (phospho Y228) antibody [E205] (ab32403)

Price and availability

304 886 ₸

Availability

Order now and get it on Wednesday March 17, 2021

Anti-delta 1 Catenin/CAS (phospho Y228) antibody [E205] (ab32403)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [E205] to delta 1 Catenin/CAS (phospho Y228)
  • Suitable for: WB, IHC-P
  • Reacts with: Human

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Overview

  • Product name

    Anti-delta 1 Catenin/CAS (phospho Y228) antibody [E205]
    See all delta 1 Catenin/CAS primary antibodies
  • Description

    Rabbit monoclonal [E205] to delta 1 Catenin/CAS (phospho Y228)
  • Host species

    Rabbit
  • Specificity

    This antibody only detects delta 1 Catenin/CAS phosphorylated on Tyrosine 228.

  • Tested Applications & Species

    Application Species
    WB
    Human
    See all applications and species data
  • Immunogen

    Synthetic peptide corresponding to Human delta 1 Catenin/CAS (phospho Y228).

  • Positive control

    • WB: HeLa whole cell lysate (ab150035). IHC-P: Breast carcinoma.
  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 49% PBS, 50% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...
  • Clonality

    Monoclonal
  • Clone number

    E205
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Cytoskeleton / ECM
    • Cytoskeleton
    • Microfilaments
    • Actin etc
    • Catenins
    • Stem Cells
    • Signaling Pathways
    • Wnt
    • Cytoplasmic

Images

  • Western blot - Anti-delta 1 Catenin/CAS (phospho Y228) antibody [E205] (ab32403)
    Western blot - Anti-delta 1 Catenin/CAS (phospho Y228) antibody [E205] (ab32403)
    All lanes : Anti-delta 1 Catenin/CAS (phospho Y228) antibody [E205] (ab32403)

    Lane 1 : HeLa cell lysate, untreated.
    Lane 2 : HeLa cell lysate, treated with Pervanandate.
    Lane 3 : HeLa cell lysate, treated with Phosphatase.

    Predicted band size: 108 kDa
    Observed band size: 115 kDa
    why is the actual band size different from the predicted?

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-delta 1 Catenin/CAS (phospho Y228) antibody [E205] (ab32403)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-delta 1 Catenin/CAS (phospho Y228) antibody [E205] (ab32403)

    Ab32403, at a 1/50 dilution, staining delta 1 Catenin/CAS in paraffin embedded breast carcinoma tissue sections by Immunohistochemistry.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Anti-delta 1 Catenin/CAS (phospho Y228) antibody [E205] (ab32403)
    Anti-delta 1 Catenin/CAS (phospho Y228) antibody [E205] (ab32403)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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