Antibodies, Proteins, Kits and Reagents for Life Science

331 689 ₸ Product size

100 µl 331 689 ₸

Order now and get it on Tuesday March 09, 2021

Anti-IMP3 antibody [EPR12021-114] (ab179807)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR12021-114] to IMP3
Suitable for: WB, IHC-P
Knockout validated
Reacts with: Human

Product name

Anti-IMP3 antibody [EPR12021-114]
See all IMP3 primary antibodies
Description

Rabbit monoclonal [EPR12021-114] to IMP3
Host species

Rabbit
Tested Applications & Species

Application Species
IHC-P
Human

WB
Human

See all applications and species data
Immunogen

Recombinant fragment within Human IMP3 aa 1-200. The exact sequence is proprietary.
Database link: O00425
Positive control
- IHC-P: Human lung cancer and fetal kidney tissues. WB: HeLa, 293T and BxPC-3 cell lysates. HeLa, HEK-293 and BxPC-3 whole cell lysates.
General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Application	Species
IHC-P	Human
WB	Human

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR12021-114
Isotype

IgG
Research areas

Western blot - Anti-IMP3 antibody [EPR12021-114] (ab179807)

Lanes 1-2 : Anti-IMP3 antibody [EPR12021-114] (ab179807) at 1/1000 dilution (Purified)
Lane 3 : Anti-IMP3 antibody [EPR12021-114] (ab179807) at 1/10000 dilution

Lane 1 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate
Lane 2 : HEK-293 (Human embryonic kidney epithelial cell) whole cell lysate
Lane 3 : BxPC-3 (Human pancreas adenocarcinoma epithelial cell) whole cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
Lanes 1-2 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Lane 3 : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/20000 dilution

Predicted band size: 63 kDa
Observed band size: 69 kDa
why is the actual band size different from the predicted?
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IMP3 antibody [EPR12021-114] (ab179807)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human lung cancer tissue sections labeling IMP3 with purified ab179807 at 1/1000 dilution (0.10 µg/mL). Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0). Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
Western blot - Anti-IMP3 antibody [EPR12021-114] (ab179807)

Lane 1: Wild-type HAP1 whole cell lysate (20 µg)
Lane 2: IGF2BP3 (IMP3) knockout HAP1 whole cell lysate (20 µg)
Lane 3: HeLa whole cell lysate (20 µg)

Lanes 1 - 3: Merged signal (red and green). Green - Unpurified ab179807 observed at 70 kDa. Red - loading control, ab9484, observed at 37 kDa.

Unpurified ab179807 was shown to specifically react with IGF2BP3 (IMP3) in wild-type HAP1 cells as signal was lost in IGF2BP3 (IMP3) knockout cells. Wild-type and IGF2BP3 (IMP3) knockout samples were subjected to SDS-PAGE. Ab179807 and ab9484 (Mouse anti GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/20,000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preabsorbed (ab216776) secondary antibodies at 1/20,000 dilution for 1 hour at room temperature before imaging.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IMP3 antibody [EPR12021-114] (ab179807)

Unpurified ab179807 at 1:100 staining IMP3 antibody in human lung cancer tissue by immunohistochemistry (FFPE). Immunohistochemical analysis of paraffin-embedded human lung cancer tissue labeling IMP3 with ab179807 at 1/100 dilution. Counter stained with hematoxylin.

Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
Western blot - Anti-IMP3 antibody [EPR12021-114] (ab179807)

All lanes : Anti-IMP3 antibody [EPR12021-114] (ab179807) at 1/1000 dilution (unpurified)

Lane 1 : HeLa cell lysate
Lane 2 : 293T cell lysate
Lane 3 : BxPC-3 cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 63 kDa
Observed band size: 69 kDa why is the actual band size different from the predicted?
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IMP3 antibody [EPR12021-114] (ab179807)

Unpurified ab179807 at 1:100 staining IMP3 antibody in human fetal kidney tissue by immunohistochemistry (FFPE). Immunohistochemical analysis of paraffin-embedded human fetal kidney tissue labeling IMP3 with ab179807 at 1/100 dilution. Counter stained with hematoxylin.

Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IMP3 antibody [EPR12021-114] (ab179807)

Negative control: Immunohistochemical analysis of paraffin-embedded human adult kidney tissue labeling IMP3 with unpurified ab179807 at 1/100 dilution. Counter stained with hematoxylin.

Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
Anti-IMP3 antibody [EPR12021-114] (ab179807)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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