Antibodies, Proteins, Kits and Reagents for Life Science

338 390 ₸ Product size

100 µl 338 390 ₸

Order now and get it on Wednesday March 10, 2021

Anti-IMP3 antibody [EPR12022] (ab177942)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR12022] to IMP3
Suitable for: WB, ICC/IF, IP
Knockout validated
Reacts with: Human

Product name

Anti-IMP3 antibody [EPR12022]
See all IMP3 primary antibodies
Description

Rabbit monoclonal [EPR12022] to IMP3
Host species

Rabbit

Tested Applications & Species

Application	Species
ICC/IF	Human
IP	Human
WB	Human

See all applications and species data

Immunogen

Recombinant fragment within Human IMP3. The exact sequence is proprietary.
Database link: O00425
Positive control
- HeLa, 293T, K562 and HT-29 cell lysates; HeLa cells.
General notes
Mouse: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
Concentration information loading...
Purity

Tissue culture supernatant
Clonality

Monoclonal
Clone number

EPR12022
Isotype

IgG
Research areas

Western blot - Anti-IMP3 antibody [EPR12022] (ab177942)

Lane 1: Wild-type HAP1 whole cell lysate (20 µg)
Lane 2: IGF2BP3 (IMP3) knockout HAP1 whole cell lysate (20 µg)
Lane 3: HeLa whole cell lysate (20 µg)
Lane 4: HEK293 whole cell lysate (20 µg)

Lanes 1 - 4: Merged signal (red and green). Green - ab177942 observed at 70 kDa. Red - loading control, ab9484, observed at 37 kDa.

ab177942 was shown to specifically react with IGF2BP3 (IMP3) in wild-type HAP1 cells as signal was lost in IGF2BP3 (IMP3) knockout cells. Wild-type and IGF2BP3 (IMP3) knockout samples were subjected to SDS-PAGE. Ab177942 and ab9484 (Mouse anti GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/20,000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preabsorbed (ab216776) secondary antibodies at 1/20,000 dilution for 1 hour at room temperature before imaging.
Immunocytochemistry/ Immunofluorescence - Anti-IMP3 antibody [EPR12022] (ab177942)

Immunofluorescent analysis of HeLa cells labeling IMP3 with ab177942 at 1/50 dilution (green). DAPI nuclear staining (blue).
Immunoprecipitation - Anti-IMP3 antibody [EPR12022] (ab177942)

Western blot analysis on immunoprecipitation pellet from 293T cell lysate using ab177942.
Western blot - Anti-IMP3 antibody [EPR12022] (ab177942)

All lanes : Anti-IMP3 antibody [EPR12022] (ab177942) at 1/1000 dilution

Lane 1 : Hela cell lysate
Lane 2 : 293T cell lysate
Lane 3 : K562 cell lysate
Lane 4 : HT-29 cell lysate

Lysates/proteins at 10 µg per lane.

Predicted band size: 63 kDa
Observed band size: 69 kDa
why is the actual band size different from the predicted?
Anti-IMP3 antibody [EPR12022] (ab177942)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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