Antibodies, Proteins, Kits and Reagents for Life Science

334 369 ₸ Product size

100 µl 334 369 ₸

Order now and get it on Tuesday March 09, 2021

HRP Anti-IMP3 antibody [EPR12021] (ab208869)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
HRP Rabbit monoclonal [EPR12021] to IMP3
Suitable for: WB
Knockout validated
Reacts with: Human
Conjugation: HRP

Product name

HRP Anti-IMP3 antibody [EPR12021]
See all IMP3 primary antibodies
Description

HRP Rabbit monoclonal [EPR12021] to IMP3
Host species

Rabbit
Conjugation

HRP
Tested Applications & Species

Application Species
WB
Human

See all applications and species data
Immunogen

Recombinant fragment within Human IMP3. The exact sequence is proprietary.
Database link: O00425
Positive control
- WB: HeLa, HEK293 cell lysates
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Application	Species
WB	Human

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark.
Storage buffer

pH: 7.40
Preservative: 0.1% 10% Proclin 300 Solution
Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR12021
Isotype

IgG
Research areas

Western blot - HRP Anti-IMP3 antibody [EPR12021] (ab208869)

All lanes : HRP Anti-IMP3 antibody [EPR12021] (ab208869) at 1/5000 dilution

Lane 1 : Wild-type HAP1 whole cell lysate
Lane 2 : IGF2BP3 (IMP3) knockout HAP1 whole cell lysate

Lysates/proteins at 20 µg per lane.

Predicted band size: 63 kDa
Observed band size: 70 kDa
why is the actual band size different from the predicted?

Exposure time: 3 minutes

ab208869 was shown to specifically react with IMP3 in wild-type HAP1 cells as signal was lost in IGF2BP3 (IMP3) knockout cells. Wild-type and IGF2BP3 (IMP3) knockout samples were subjected to SDS-PAGE. Ab208869 and ab184095 (Mouse monoclonal [mAbcam 9484] to GAPDH - Loading Control (Alexa Fluor^® 680) loading control) were incubated overnight at 4°C at 1/5000 dilution and 1/20000 dilution respectively. The loading control was imaged using the Licor Odyssey CLx prior to blots being developed with ECL technique.
Western blot - HRP Anti-IMP3 antibody [EPR12021] (ab208869)

All lanes : HRP Anti-IMP3 antibody [EPR12021] (ab208869) at 1/5000 dilution

Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate

Lysates/proteins at 10 µg per lane.

Developed using the ECL technique.

Performed under reducing conditions.

Predicted band size: 63 kDa
Observed band size: 70 kDa why is the actual band size different from the predicted?

Exposure time: 15 seconds

This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab208869 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.
HRP Anti-IMP3 antibody [EPR12021] (ab208869)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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