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Signal Transduction Protein Trafficking Vesicle Transport Regulation

Anti-EIF2S1 (phospho S49) antibody (ab131489)

Anti-EIF2S1 (phospho S49) antibody (ab131489)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Rabbit polyclonal to EIF2S1 (phospho S49)
  • Suitable for: WB, IHC-P, ICC/IF
  • Reacts with: Human
  • Isotype: IgG

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Overview

  • Product name

    Anti-EIF2S1 (phospho S49) antibody
    See all EIF2S1 primary antibodies
  • Description

    Rabbit polyclonal to EIF2S1 (phospho S49)
  • Host species

    Rabbit
  • Specificity

    ab131489 detects endogenous levels of EIF2S1 only when phosphorylated at Serine 49.
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide around phosphorylation site of serine 49 (L-L-S(p)-E-L) derived from Human EIF2S1 (NP_004085.1)

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive control

    • HeLa cell lysate Human breast carcinoma tissue
  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: 49% PBS, 50% Glycerol (glycerin, glycerine), 0.88% Sodium chloride

    PBS is without Mg2+ and Ca2+
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    ab131489 was purified by affinity-chromatography using an-epitope specific phosphopeptide. Non-phosphopeptide specific antibodies were removed by chromatography using non-phosphopeptide.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Epigenetics and Nuclear Signaling
    • DNA / RNA
    • Translation
    • Regulation
    • Epigenetics and Nuclear Signaling
    • RNAi
    • Eukaryotic Initiation factors (eIF's)

Images

  • Western blot - Anti-EIF2S1 (phospho S49) antibody (ab131489)
    Western blot - Anti-EIF2S1 (phospho S49) antibody (ab131489)
    All lanes : Anti-EIF2S1 (phospho S49) antibody (ab131489) at 1/500 dilution

    Lane 1 : Extracts of Hela cells untreated
    Lane 2 : Extracts of Hela cells treated with starvation

    Predicted band size: 36 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-EIF2S1 (phospho S49) antibody (ab131489)
    Immunocytochemistry/ Immunofluorescence - Anti-EIF2S1 (phospho S49) antibody (ab131489)
    Immunofluorescence analysis of methanol-fixed HeLa cells labelling EIF2S1 using ab131489, at 1/100 dilution.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EIF2S1 (phospho S49) antibody (ab131489)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EIF2S1 (phospho S49) antibody (ab131489)
    Immunohistochemistry analysis of Human breast cancer labelling EIF2S1 using ab131489, at 1/100 dilution (Left) or preincubated with blocking peptide (right).

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Alternative products to Anti-EIF2S1 (phospho S49) antibody (ab131489)

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