Anti-L3MBTL2 antibody (ab228586)
Key features and details
- Rabbit polyclonal to L3MBTL2
- Suitable for: WB, IP, ChIP
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-L3MBTL2 antibody
See all L3MBTL2 primary antibodies -
Description
Rabbit polyclonal to L3MBTL2 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IP, ChIPmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Cow
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Immunogen
Recombinant fragment within Human L3MBTL2 (internal sequence). The exact sequence is proprietary.
Database link: Q969R5 -
Positive control
- WB: NT2/D1 whole cell extract; mouse testis tissue extract. IP: HeLa whole cell lysate. ChIP: HeLa whole cell extract/lysate.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunoprecipitation - Anti-L3MBTL2 antibody (ab228586)
L3MBTL2 was immunoprecipitated from 1 mg of HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate with 2.5 μg ab228586. Western blot was performed from the immunoprecipitate using ab228586 at 1/1000 dilution.
Lane 1: HeLa whole cell lysate 40 μg (Input).
Lane 2: Control pre-immune rabbit IgG IP in HeLa whole cell lysate.
Lane 3: ab228586 IP in HeLa whole cell lysate.
5% SDS-PAGE
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ChIP - Anti-L3MBTL2 antibody (ab228586)ChIP was performed on HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extract/lysate with 5 μg control pre-immune rabbit IgG (A) or with 5 μg ab228586 (B).
The precipitated DNA was detected by PCR with primer set targeting to the CDC7 gene locus.
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Western blot - Anti-L3MBTL2 antibody (ab228586)Anti-L3MBTL2 antibody (ab228586) at 1/500 dilution + NT2/D1 (human embryonal testis carcinoma cell line) whole cell extract at 30 µg
Developed using the ECL technique.
Predicted band size: 79 kDa7.5% SDS-PAGE
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Western blot - Anti-L3MBTL2 antibody (ab228586)Anti-L3MBTL2 antibody (ab228586) at 1/500 dilution + Mouse testis tissue extract at 50 µg
Developed using the ECL technique.
Predicted band size: 79 kDa7.5% SDS-PAGE
